TY - JOUR
T1 - Elucidation of n-butyl benzyl phthalate biodegradation using high-performance liquid chromatography and gas chromatography-mass spectrometry
AU - Xu, Xiang Rong
AU - Li, Hua Bin
AU - Gu, Ji Dong
PY - 2006/10
Y1 - 2006/10
N2 - n-Butyl benzyl phthalate (BBP) is an endocrinedisrupting chemical. A bacterium species capable of using BBP as the sole source of carbon and energy was isolated from mangrove sediment. Effects of BBP concentration, pH, temperature, and salinity on BBP biodegradation were studied. The optimum pH, temperature, and salinity for the BBP biodegradation were 7.0, 37°C, and 15 g L-1, respectively. BBP was completely degraded within 6 days under optimum conditions, and the biodegradation of BBP could be fitted to a first-order kinetic model. The major metabolites of BBP biodegradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid, and benzoic acid by using high-performance liquid chromatography and gas chromatography-mass spectrometry. A preliminary metabolic pathway was proposed for the biodegradation of BBP.
AB - n-Butyl benzyl phthalate (BBP) is an endocrinedisrupting chemical. A bacterium species capable of using BBP as the sole source of carbon and energy was isolated from mangrove sediment. Effects of BBP concentration, pH, temperature, and salinity on BBP biodegradation were studied. The optimum pH, temperature, and salinity for the BBP biodegradation were 7.0, 37°C, and 15 g L-1, respectively. BBP was completely degraded within 6 days under optimum conditions, and the biodegradation of BBP could be fitted to a first-order kinetic model. The major metabolites of BBP biodegradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid, and benzoic acid by using high-performance liquid chromatography and gas chromatography-mass spectrometry. A preliminary metabolic pathway was proposed for the biodegradation of BBP.
KW - Biodegradation
KW - Gas chromatography-mass spectrometry
KW - High-performance liquid chromatography
KW - N-Butyl benzyl phthalate
UR - http://www.scopus.com/inward/record.url?scp=84871131412&partnerID=8YFLogxK
U2 - 10.1007/s00216-006-0627-7
DO - 10.1007/s00216-006-0627-7
M3 - 文章
C2 - 16871373
AN - SCOPUS:84871131412
SN - 1618-2642
VL - 386
SP - 370
EP - 375
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 2
ER -