Elucidating the Role of Biofilm-Forming Microbial Communities in Fermentative Biohydrogen Process: An Overview

Patrick T. Sekoai*, Viren Chunilall, Bruce Sithole, Olivier Habimana, Sizwe Ndlovu, Obinna T. Ezeokoli, Pooja Sharma, Kelvin O. Yoro

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

3 Scopus citations


Amongst the biofuels described in the literature, biohydrogen has gained heightened attention over the past decade due to its remarkable properties. Biohydrogen is a renewable form of H2 that can be produced under ambient conditions and at a low cost from biomass residues. Innovative approaches are continuously being applied to overcome the low process yields and pave the way for its scalability. Since the process primarily depends on the biohydrogen-producing bacteria, there is a need to acquire in-depth knowledge about the ecology of the various assemblages participating in the process, establishing effective bioaugmentation methods. This work provides an overview of the biofilm-forming communities during H2 production by mixed cultures and the synergistic associations established by certain species during H2 production. The strategies that enhance the growth of biofilms within the H2 reactors are also discussed. A short section is also included, explaining techniques used for examining and studying these biofilm structures. The work concludes with some suggestions that could lead to breakthroughs in this area of research.

Original languageEnglish
Article number1924
Issue number10
StatePublished - Oct 2022


  • biofilms
  • biofuels
  • biohydrogen
  • fermentation
  • renewable energy


Dive into the research topics of 'Elucidating the Role of Biofilm-Forming Microbial Communities in Fermentative Biohydrogen Process: An Overview'. Together they form a unique fingerprint.

Cite this