Dominant and Active Methanogens in the Production Waters From a High-Temperature Petroleum Reservoir by DNA- and RNA-Based Analysis

TY - JOUR

T1 - Dominant and Active Methanogens in the Production Waters From a High-Temperature Petroleum Reservoir by DNA- and RNA-Based Analysis

AU - Zhou, Lei

AU - Hu, Qian Qian

AU - Lu, Yu Wei

AU - Mbadinga, Serge Maurice

AU - Liu, Yi Fan

AU - Li, Xiao Xiao

AU - Wang, Biao

AU - Lv, Hongmei

AU - Liu, Jin Feng

AU - Yang, Shi Zhong

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

N1 - Publisher Copyright: © 2020 Informa UK Limited, trading as Taylor & Francis Group.

PY - 2021

Y1 - 2021

N2 - Methane metabolism in deep subsurface petroleum reservoirs is of paramount interest in the global biogeochemical cycle of carbon. Methanogenesis in such habitats is driven mostly by methanogens in syntrophic association with bacteria. In the present study, methanogenic communities in production water samples from six wells of a high-temperature petroleum reservoir were analyzed based on both genomic 16S rDNA and metabolically active 16S rRNA. The PCR-amplified mcrA gene analysis showed that Methanosaeta (26%) and Methanomassiliicoccus (56%) were separately the dominant members in sample W2_71 and W9_18 at the DNA level. In comparison, the RNA-based analysis showed that Methanosaeta (63 ∼ 83%) followed by Methanolinea (16 ∼ 23%) were the most active methanogens, which were different from the communities of genomic DNA. While several lines of studies indicated that CO2-reducing methanogens of the genus Methanothermobacter was the most frequently detected phylotype in deep-subsurface petroleum reservoirs. Redundancy analysis (RDA) showed the possible correlation between active methanogens (Methanosaeta) and environmental factors (CO32-). The datasets indicated the importance of investigating methanogenic community by integrating DNA- and RNA-based approaches. These results provide new insight into active methanogens in high-temperature petroleum reservoirs, and promote a comprehensive understanding of methanogens as well as their potential methanogenic pathways in such environments.

AB - Methane metabolism in deep subsurface petroleum reservoirs is of paramount interest in the global biogeochemical cycle of carbon. Methanogenesis in such habitats is driven mostly by methanogens in syntrophic association with bacteria. In the present study, methanogenic communities in production water samples from six wells of a high-temperature petroleum reservoir were analyzed based on both genomic 16S rDNA and metabolically active 16S rRNA. The PCR-amplified mcrA gene analysis showed that Methanosaeta (26%) and Methanomassiliicoccus (56%) were separately the dominant members in sample W2_71 and W9_18 at the DNA level. In comparison, the RNA-based analysis showed that Methanosaeta (63 ∼ 83%) followed by Methanolinea (16 ∼ 23%) were the most active methanogens, which were different from the communities of genomic DNA. While several lines of studies indicated that CO2-reducing methanogens of the genus Methanothermobacter was the most frequently detected phylotype in deep-subsurface petroleum reservoirs. Redundancy analysis (RDA) showed the possible correlation between active methanogens (Methanosaeta) and environmental factors (CO32-). The datasets indicated the importance of investigating methanogenic community by integrating DNA- and RNA-based approaches. These results provide new insight into active methanogens in high-temperature petroleum reservoirs, and promote a comprehensive understanding of methanogens as well as their potential methanogenic pathways in such environments.

KW - Methanogen

KW - RNA

KW - mcrA

KW - methanogenesis

KW - petroleum reservoir

UR - http://www.scopus.com/inward/record.url?scp=85092272111&partnerID=8YFLogxK

U2 - 10.1080/01490451.2020.1822958

DO - 10.1080/01490451.2020.1822958

M3 - 文章

AN - SCOPUS:85092272111

SN - 0149-0451

VL - 38

SP - 191

EP - 198

JO - Geomicrobiology Journal

JF - Geomicrobiology Journal

IS - 3

ER -