TY - JOUR
T1 - Diversity of ammonia-oxidizing bacteria and archaea in response to different aeration rates during cattle manure composting
AU - Yan, Lei
AU - Li, Zhenguo
AU - Wang, Guoxing
AU - Gao, Yamei
AU - Wang, Yanjie
AU - Gu, Ji Dong
AU - Wang, Weidong
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Both ammonia-oxidizing bacteria (AOB) and archaea (AOA) coexisted in the process of cattle manure composting under different rates of aeration in this study. The effect of aeration rates (12, 3 and 0 L/min) on the communities of AOB and AOA in the cattle manure composting was evaluated using the molecular marker amoA gene for phylogenetic comparisons. Nitrosomonas and Nitrosospira affiliated with AOB were detected and the relative abundance of Nitrosomonas ranged from 69.1% to 100% showing its dominance in cattle manure composting. The diversity of AOA showed no significant change with the increase of aeration rates, only 2.0% of the sequences of marine cluster appeared under treatment with aeration rate of 12 L/min. Relationships between diversity of AOB and AOA and physicochemical parameters were also explored. Canonical correspondence analysis (CCA) indicated that oxygen was significantly (p < 0.05) correlated with diversity of AOB, but not with AOA. These results revealed that AOB were more important than AOA in nitrogen transformation, including nitrification during cattle manure composting under different aeration rates.
AB - Both ammonia-oxidizing bacteria (AOB) and archaea (AOA) coexisted in the process of cattle manure composting under different rates of aeration in this study. The effect of aeration rates (12, 3 and 0 L/min) on the communities of AOB and AOA in the cattle manure composting was evaluated using the molecular marker amoA gene for phylogenetic comparisons. Nitrosomonas and Nitrosospira affiliated with AOB were detected and the relative abundance of Nitrosomonas ranged from 69.1% to 100% showing its dominance in cattle manure composting. The diversity of AOA showed no significant change with the increase of aeration rates, only 2.0% of the sequences of marine cluster appeared under treatment with aeration rate of 12 L/min. Relationships between diversity of AOB and AOA and physicochemical parameters were also explored. Canonical correspondence analysis (CCA) indicated that oxygen was significantly (p < 0.05) correlated with diversity of AOB, but not with AOA. These results revealed that AOB were more important than AOA in nitrogen transformation, including nitrification during cattle manure composting under different aeration rates.
KW - Aeration
KW - Ammonia-oxidizing archaea
KW - Ammonia-oxidizing bacteria
KW - AmoA
KW - Cattle manure composting
UR - http://www.scopus.com/inward/record.url?scp=84967185892&partnerID=8YFLogxK
U2 - 10.1016/j.ecoleng.2016.05.002
DO - 10.1016/j.ecoleng.2016.05.002
M3 - 文章
AN - SCOPUS:84967185892
SN - 0925-8574
VL - 93
SP - 46
EP - 54
JO - Ecological Engineering
JF - Ecological Engineering
ER -
