TY - JOUR
T1 - Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea
AU - Cao, Huiluo
AU - Hong, Yiguo
AU - Li, Meng
AU - Gu, Ji Dong
N1 - Funding Information:
Acknowledgments This research was supported by a PhD studentship (H-L C) from The University of Hong Kong and in part by grants from Agriculture, Fisheries and Conservation Department of the Hong Kong SAR Government (J-DG), and National Natural Science Foundation of China (41076095), Knowledge Innovation Key Project of the Chinese Academy of Sciences (KZCX2-YW-QN207) (Y-GH). We would like to thank Ms. Jessie Lai and Dr Zhenye Zhao for their kindly laboratory assistances throughout this investigation at The University of Hong Kong.
PY - 2011/11
Y1 - 2011/11
N2 - In the present study the diversity and abundance of nitrifying microbes including ammonia-oxidizing archaea (AOA) and betaproteobacteria (beta-AOB) were investigated, along with the physicochemical parameters potentially affecting them, in a transect of surface sediments from the coastal margin adjacent to the Pearl River estuary to the slope in the deep South China Sea. Nitrifying microbial diversity was determined by detecting the amoA (ammonia monooxygenase subunit A) gene. An obvious community structure shift for both AOA and beta-AOB from the coastal marginal areas to the slope in the deep-sea was detected, while the OTU numbers of AOA amoA were more stable than those of the beta-AOB. The OTUs of beta-AOB increased with the distance from the coastal margin areas to the slope in the deep-sea. Beta-AOB showed lower diversity with dominant strains in a polluted area but higher diversity without dominant strains in a clean area. Moreover, the diversity of beta-AOB was correlated with pH values, while no noticeable relationships were established between AOA and physicochemical parameters. Beta-AOB was more sensitive to transect environmental variability and might be a potential indicator for environmental changes. Additionally, the surface sediments surveyed in the South China Sea harboured diverse and distinct AOA and beta-AOB phylotypes different from other environments, suggesting the endemicity of some nitrifying prokaryotes in the South China Sea.
AB - In the present study the diversity and abundance of nitrifying microbes including ammonia-oxidizing archaea (AOA) and betaproteobacteria (beta-AOB) were investigated, along with the physicochemical parameters potentially affecting them, in a transect of surface sediments from the coastal margin adjacent to the Pearl River estuary to the slope in the deep South China Sea. Nitrifying microbial diversity was determined by detecting the amoA (ammonia monooxygenase subunit A) gene. An obvious community structure shift for both AOA and beta-AOB from the coastal marginal areas to the slope in the deep-sea was detected, while the OTU numbers of AOA amoA were more stable than those of the beta-AOB. The OTUs of beta-AOB increased with the distance from the coastal margin areas to the slope in the deep-sea. Beta-AOB showed lower diversity with dominant strains in a polluted area but higher diversity without dominant strains in a clean area. Moreover, the diversity of beta-AOB was correlated with pH values, while no noticeable relationships were established between AOA and physicochemical parameters. Beta-AOB was more sensitive to transect environmental variability and might be a potential indicator for environmental changes. Additionally, the surface sediments surveyed in the South China Sea harboured diverse and distinct AOA and beta-AOB phylotypes different from other environments, suggesting the endemicity of some nitrifying prokaryotes in the South China Sea.
KW - Ammonia monooxygenase subunit A (amoA)
KW - Ammonia-oxidizing archaea
KW - Ammonia-oxidizing bacteria
KW - South China Sea
UR - http://www.scopus.com/inward/record.url?scp=80054098914&partnerID=8YFLogxK
U2 - 10.1007/s10482-011-9610-1
DO - 10.1007/s10482-011-9610-1
M3 - 文章
C2 - 21717206
AN - SCOPUS:80054098914
SN - 0003-6072
VL - 100
SP - 545
EP - 556
JO - Antonie van Leeuwenhoek
JF - Antonie van Leeuwenhoek
IS - 4
ER -