TY - JOUR
T1 - Direct cryogenic-temperature transmission electron microscopy imaging of phospholipid aggregates in soybean oil
AU - Danino, Dganit
AU - Gupta, Rajan
AU - Satyavolu, Jagannadh
AU - Talmon, Yeshayahu
N1 - Funding Information:
This work was supported in part by a grant from the U.S. Department of Commerce, National Institute of Standards and Technology (Cooperative Agreement Number 70NANB8H4028). The cryo-TEM work was performed at the “Cryo-TEM Hannah and George Krumholz Laboratory for Advanced Microscopy” at the Technion, part of the “Technion Project on Complex Fluids.” We thank Judith Schmidt and Berta Shdemati for excellent technical assistance.
PY - 2002
Y1 - 2002
N2 - Cryogenic-temperature transmission electron microscopy (cryo-TEM) was fine-tuned to make it applicable to the study of a natural oil-based system. While liquid ethane, the cryogen of choice for specimen preparation, was found to dissolve the oil, we observed that the triglycerides found in many natural oils serve as cryoprotectants that allow vitrification of such oil systems in liquid nitrogen, a cryogen that provides relatively low cooling rates. Using the modified technique combined with digital imaging by a cooled CCD camera, we were able to directly visualize inverse micellar and liquid-crystalline aggregates formed in the soybean oil/soybean phospholipids/water/hexane system, which is commercially important in soybean oil processing. The method developed here should be widely applicable to other organic solvent-based systems.
AB - Cryogenic-temperature transmission electron microscopy (cryo-TEM) was fine-tuned to make it applicable to the study of a natural oil-based system. While liquid ethane, the cryogen of choice for specimen preparation, was found to dissolve the oil, we observed that the triglycerides found in many natural oils serve as cryoprotectants that allow vitrification of such oil systems in liquid nitrogen, a cryogen that provides relatively low cooling rates. Using the modified technique combined with digital imaging by a cooled CCD camera, we were able to directly visualize inverse micellar and liquid-crystalline aggregates formed in the soybean oil/soybean phospholipids/water/hexane system, which is commercially important in soybean oil processing. The method developed here should be widely applicable to other organic solvent-based systems.
KW - Cryo-TEM
KW - Inverse micelles
KW - Lyotropic liquid crystals
KW - Soybean oil
UR - http://www.scopus.com/inward/record.url?scp=0036353073&partnerID=8YFLogxK
U2 - 10.1006/jcis.2002.8229
DO - 10.1006/jcis.2002.8229
M3 - 文章
C2 - 16290584
AN - SCOPUS:0036353073
SN - 0021-9797
VL - 249
SP - 180
EP - 186
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
IS - 1
ER -