Dimethyl phthalate ester degradation by two planktonic and immobilized bacterial consortia

TY - JOUR

T1 - Dimethyl phthalate ester degradation by two planktonic and immobilized bacterial consortia

AU - Wang, Yingying

AU - Fan, Yanzhen

AU - Gu, Ji Dong

N1 - Funding Information: This research was supported by a Grant (ITS/276/00) from the Innovative Technology Commission of Hong Kong SAR and industrial partnerships with Kou Hing Hong Scientific Supplies and Peako Engineering Co. We would like to thank the reviewers for their comments and Jessie Lai for technical laboratory support.

PY - 2004/3

Y1 - 2004/3

N2 - Degradation of the plasticizer ortho-dimethyl phthalate ester (DMPE) was investigated using two reconstituted consortia under aerobic conditions. The consortia consisting of two and three bacterial cultures showed capability in the complete degradation of DMPE in less than 5 days at an initial concentration of 400 mg l-1. Consortium I, comprised of Pseudomonas fluorescens, P. aureofaciens and Sphingomonas paucimobilis, appeared to be more effective in degradation than Consortium II consisting of Xanthomonas maltophilia and S. paucimobilis. The Gompertz function and the Richards function were modified and used to fit the DMPE degradation data and the results indicated that the modified Richards model was always better than the modified Gompertz model in fitting the experimental data. In addition, microorganisms capable of degrading DMPE were subsequently immobilized on the microporous hollow fibres using calcium alginate entrapment techniques. The efficiency of DMPE degradation by the immobilized bacteria of Consortium I was compared with the batch culture system. The results showed that immobilization of microorganisms could further improve the rates of degradation. Our data suggest that microbial degradation of DMPE can be described by the modified Richards model and the plasticizer DMPE can be mineralized by consortia of bacteria either in planktonic culture or immobilized on surfaces.

AB - Degradation of the plasticizer ortho-dimethyl phthalate ester (DMPE) was investigated using two reconstituted consortia under aerobic conditions. The consortia consisting of two and three bacterial cultures showed capability in the complete degradation of DMPE in less than 5 days at an initial concentration of 400 mg l-1. Consortium I, comprised of Pseudomonas fluorescens, P. aureofaciens and Sphingomonas paucimobilis, appeared to be more effective in degradation than Consortium II consisting of Xanthomonas maltophilia and S. paucimobilis. The Gompertz function and the Richards function were modified and used to fit the DMPE degradation data and the results indicated that the modified Richards model was always better than the modified Gompertz model in fitting the experimental data. In addition, microorganisms capable of degrading DMPE were subsequently immobilized on the microporous hollow fibres using calcium alginate entrapment techniques. The efficiency of DMPE degradation by the immobilized bacteria of Consortium I was compared with the batch culture system. The results showed that immobilization of microorganisms could further improve the rates of degradation. Our data suggest that microbial degradation of DMPE can be described by the modified Richards model and the plasticizer DMPE can be mineralized by consortia of bacteria either in planktonic culture or immobilized on surfaces.

KW - Bacterial consortium

KW - Biofilm

KW - Degradation

KW - Endocrine-disrupting chemicals

KW - Immobilization

KW - Phthalate dimethyl ester

KW - Plasticizer

UR - http://www.scopus.com/inward/record.url?scp=1242277886&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2003.10.005

DO - 10.1016/j.ibiod.2003.10.005

M3 - 文章

AN - SCOPUS:1242277886

SN - 0964-8305

VL - 53

SP - 93

EP - 101

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

IS - 2

ER -