Comparison of different treatment methods for protein solubilisation from waste activated sludge

Keke Xiao; Yun Chen; Xie Jiang; Wan Yi Seow; Chao He; Yao Yin; Yan Zhou

doi:10.1016/j.watres.2017.06.024

Comparison of different treatment methods for protein solubilisation from waste activated sludge

Keke Xiao, Yun Chen, Xie Jiang, Wan Yi Seow, Chao He, Yao Yin, Yan Zhou^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

Biomaterials recovery from wasted activated sludge has become an increasing interesting research topic. The purpose of this study was to systemically evaluate different sludge disintegration methods (ultrasonic, alkaline, and thermal treatments) for protein solubilisation from waste activated sludge (WAS). Compared to control without treatment, the soluble protein concentration increased by 11, 23 and 12 times under the optimal treatment conditions (ultrasonic treatment of 1 W mL⁻¹, alkaline treatment of pH 12 and thermal treatment at 80 °C). The increased soluble protein were significantly correlated with the release of total organic carbon (TOC), total dissolved nitrogen (TDN) and total organic nitrogen (TON) in soluble EPS, and the degradation of above parameters in tightly bound EPS. For all sludge samples treated by various methods, tyrosine-like protein with molecular weight less than 20 kDa predominated, and alkaline treatment at pH 12 showed the highest protein dominance. Further surface analysis of sludge by X-ray photoelectron spectroscopy indicated this might be related with the significant protein-N conversion occurred at pH 12. The economic analysis indicated alkaline treatment at pH 12 was economically feasible with a net saving of 25.57 USD per ton wet sludge compared to conventional sludge treatment and disposal method.

Original language	English
Pages (from-to)	492-502
Number of pages	11
Journal	Water Research
Volume	122
DOIs	https://doi.org/10.1016/j.watres.2017.06.024
State	Published - 2017
Externally published	Yes

Keywords

Extracellular polymeric substances
Molecular weight distribution
N-containing component
Protein solubilisation
Treatments

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title = "Comparison of different treatment methods for protein solubilisation from waste activated sludge",

abstract = "Biomaterials recovery from wasted activated sludge has become an increasing interesting research topic. The purpose of this study was to systemically evaluate different sludge disintegration methods (ultrasonic, alkaline, and thermal treatments) for protein solubilisation from waste activated sludge (WAS). Compared to control without treatment, the soluble protein concentration increased by 11, 23 and 12 times under the optimal treatment conditions (ultrasonic treatment of 1 W mL−1, alkaline treatment of pH 12 and thermal treatment at 80 °C). The increased soluble protein were significantly correlated with the release of total organic carbon (TOC), total dissolved nitrogen (TDN) and total organic nitrogen (TON) in soluble EPS, and the degradation of above parameters in tightly bound EPS. For all sludge samples treated by various methods, tyrosine-like protein with molecular weight less than 20 kDa predominated, and alkaline treatment at pH 12 showed the highest protein dominance. Further surface analysis of sludge by X-ray photoelectron spectroscopy indicated this might be related with the significant protein-N conversion occurred at pH 12. The economic analysis indicated alkaline treatment at pH 12 was economically feasible with a net saving of 25.57 USD per ton wet sludge compared to conventional sludge treatment and disposal method.",

keywords = "Extracellular polymeric substances, Molecular weight distribution, N-containing component, Protein solubilisation, Treatments",

author = "Keke Xiao and Yun Chen and Xie Jiang and Seow, {Wan Yi} and Chao He and Yao Yin and Yan Zhou",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2017",

doi = "10.1016/j.watres.2017.06.024",

language = "英语",

volume = "122",

pages = "492--502",

journal = "Water Research",

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T1 - Comparison of different treatment methods for protein solubilisation from waste activated sludge

AU - Xiao, Keke

AU - Chen, Yun

AU - Jiang, Xie

AU - Seow, Wan Yi

AU - He, Chao

AU - Yin, Yao

AU - Zhou, Yan

PY - 2017

Y1 - 2017

N2 - Biomaterials recovery from wasted activated sludge has become an increasing interesting research topic. The purpose of this study was to systemically evaluate different sludge disintegration methods (ultrasonic, alkaline, and thermal treatments) for protein solubilisation from waste activated sludge (WAS). Compared to control without treatment, the soluble protein concentration increased by 11, 23 and 12 times under the optimal treatment conditions (ultrasonic treatment of 1 W mL−1, alkaline treatment of pH 12 and thermal treatment at 80 °C). The increased soluble protein were significantly correlated with the release of total organic carbon (TOC), total dissolved nitrogen (TDN) and total organic nitrogen (TON) in soluble EPS, and the degradation of above parameters in tightly bound EPS. For all sludge samples treated by various methods, tyrosine-like protein with molecular weight less than 20 kDa predominated, and alkaline treatment at pH 12 showed the highest protein dominance. Further surface analysis of sludge by X-ray photoelectron spectroscopy indicated this might be related with the significant protein-N conversion occurred at pH 12. The economic analysis indicated alkaline treatment at pH 12 was economically feasible with a net saving of 25.57 USD per ton wet sludge compared to conventional sludge treatment and disposal method.

AB - Biomaterials recovery from wasted activated sludge has become an increasing interesting research topic. The purpose of this study was to systemically evaluate different sludge disintegration methods (ultrasonic, alkaline, and thermal treatments) for protein solubilisation from waste activated sludge (WAS). Compared to control without treatment, the soluble protein concentration increased by 11, 23 and 12 times under the optimal treatment conditions (ultrasonic treatment of 1 W mL−1, alkaline treatment of pH 12 and thermal treatment at 80 °C). The increased soluble protein were significantly correlated with the release of total organic carbon (TOC), total dissolved nitrogen (TDN) and total organic nitrogen (TON) in soluble EPS, and the degradation of above parameters in tightly bound EPS. For all sludge samples treated by various methods, tyrosine-like protein with molecular weight less than 20 kDa predominated, and alkaline treatment at pH 12 showed the highest protein dominance. Further surface analysis of sludge by X-ray photoelectron spectroscopy indicated this might be related with the significant protein-N conversion occurred at pH 12. The economic analysis indicated alkaline treatment at pH 12 was economically feasible with a net saving of 25.57 USD per ton wet sludge compared to conventional sludge treatment and disposal method.

KW - Extracellular polymeric substances

KW - Molecular weight distribution

KW - N-containing component

KW - Protein solubilisation

KW - Treatments

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U2 - 10.1016/j.watres.2017.06.024

DO - 10.1016/j.watres.2017.06.024

M3 - 文章

C2 - 28624732

AN - SCOPUS:85020800512

SN - 0043-1354

VL - 122

SP - 492

EP - 502

JO - Water Research

JF - Water Research

ER -

Comparison of different treatment methods for protein solubilisation from waste activated sludge

Abstract

Keywords

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