Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda

Y. P. Tan; Q. Lin; X. H. Wang; S. Joshi; C. L. Hew; K. Y. Leung

doi:10.1128/IAI.70.11.6475-6480.2002

Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda

Y. P. Tan, Q. Lin, X. H. Wang, S. Joshi, C. L. Hew, K. Y. Leung^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.

Original language	English
Pages (from-to)	6475-6480
Number of pages	6
Journal	Infection and Immunity
Volume	70
Issue number	11
DOIs	https://doi.org/10.1128/IAI.70.11.6475-6480.2002
State	Published - Nov 2002
Externally published	Yes

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10.1128/IAI.70.11.6475-6480.2002

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abstract = "A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.",

author = "Tan, {Y. P.} and Q. Lin and Wang, {X. H.} and S. Joshi and Hew, {C. L.} and Leung, {K. Y.}",

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AU - Tan, Y. P.

AU - Lin, Q.

AU - Wang, X. H.

AU - Joshi, S.

AU - Hew, C. L.

AU - Leung, K. Y.

PY - 2002/11

Y1 - 2002/11

N2 - A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.

AB - A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.

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U2 - 10.1128/IAI.70.11.6475-6480.2002

DO - 10.1128/IAI.70.11.6475-6480.2002

M3 - 文章

C2 - 12379732

AN - SCOPUS:0036840350

SN - 0019-9567

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SP - 6475

EP - 6480

JO - Infection and Immunity

JF - Infection and Immunity

IS - 11

ER -

Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda

Abstract

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