Cloning of the organophosphorus pesticide hydrolase gene clusters of seven degradative bacteria isolated from a methyl parathion contaminated site and evidence of their horizontal gene transfer

TY - JOUR

T1 - Cloning of the organophosphorus pesticide hydrolase gene clusters of seven degradative bacteria isolated from a methyl parathion contaminated site and evidence of their horizontal gene transfer

AU - Zhang, Ruifu

AU - Cui, Zhongli

AU - Zhang, Xiaozhou

AU - Jiang, Jiandong

AU - Gu, Ji Dong

AU - Li, Shunpeng

N1 - Funding Information: This work was supported by grants 30400014 and 680600016 from the Chinese National Natural Science Foundation, and by the Chinese National Programs for High Technology Research and Development (2003AA241150).

PY - 2006/10

Y1 - 2006/10

N2 - Seven organophosphorus pesticide-degrading bacteria harboring the methyl parathion degrading (mpd) gene were isolated from a methyl parathion contaminated site. In this study, the 4.7 kb mpd gene cluster, conserved in all seven bacteria capable of degrading methyl parathion, was cloned and further analysis revealed that this cluster contained five ORFs and the mpd gene was associated with a mobile element, IS6100. In addition to mpd gene ORF and tnpA ORF, three other ORFs showed high homology to the permease component of ABC-type transport system, the general secretion pathway protein B, and the RNA polymerase sigma 70 factor, respectively. The mpd genes of these 7 strains were subcloned and expressed in E. coli, SDS-PAGE and zymogram analysis showed that two expression products of mpd genes in E. coli were found, but the one without signal peptide showed the hydrolytic activities. Our evidences collectively suggest that mpd gene cluster may be disseminated through horizontal gene transfer based on phylogenetic analysis of the cluster and their host bacterial strains, and comparisons of GC content of the cluster and respective host's chromosome.

AB - Seven organophosphorus pesticide-degrading bacteria harboring the methyl parathion degrading (mpd) gene were isolated from a methyl parathion contaminated site. In this study, the 4.7 kb mpd gene cluster, conserved in all seven bacteria capable of degrading methyl parathion, was cloned and further analysis revealed that this cluster contained five ORFs and the mpd gene was associated with a mobile element, IS6100. In addition to mpd gene ORF and tnpA ORF, three other ORFs showed high homology to the permease component of ABC-type transport system, the general secretion pathway protein B, and the RNA polymerase sigma 70 factor, respectively. The mpd genes of these 7 strains were subcloned and expressed in E. coli, SDS-PAGE and zymogram analysis showed that two expression products of mpd genes in E. coli were found, but the one without signal peptide showed the hydrolytic activities. Our evidences collectively suggest that mpd gene cluster may be disseminated through horizontal gene transfer based on phylogenetic analysis of the cluster and their host bacterial strains, and comparisons of GC content of the cluster and respective host's chromosome.

KW - Degradation

KW - Horizontal gene transfer

KW - Organophosphorus hydrolase gene cluster

KW - Signal peptide

UR - http://www.scopus.com/inward/record.url?scp=33748657366&partnerID=8YFLogxK

U2 - 10.1007/s10532-005-9018-6

DO - 10.1007/s10532-005-9018-6

M3 - 文章

C2 - 16477356

AN - SCOPUS:33748657366

SN - 0923-9820

VL - 17

SP - 465

EP - 472

JO - Biodegradation

JF - Biodegradation

IS - 5

ER -