TY - JOUR
T1 - Characterization of genetic variation and relationships among choix germplasm accessions using rapd markers
AU - Li, Xinhai
AU - Huang, Yiqin
AU - Li, Jiansheng
AU - Corke, Harold
N1 - Funding Information:
This work was supported by the International Fund of Science, the University of Hong Kong Committee on Research and Conference Grants, and the National Natural Science Fund of China. We thank Dr. Mei Sun for critical review of the manuscript.
PY - 2001
Y1 - 2001
N2 - Choix, a plant in the tribe Maydeae of the grass family, has been cultivated in Asia for several thousand years. It is a potential gene resource for improvement of other cereal crops because of its nutritional value and tolerance to stress. Genetic variation and relationships among 21 Choix lachryma-jobi L. accessions were characterized by random amplified polymorphic DNA (RAPD) markers. A total of 205 DNA fragments across all materials were amplified with 31 random primers, averaging 6.61 per primer. Among amplified fragments, 115 showed polymorphism averaging 3.71 per primer. Of amplified markers, 56.1% were polymorphic, indicating considerable variation at the DNA level among these accessions. Some fragments were accession-specific. Pair-wise genetic similarity (GS) among 21 accessions ranged from 0.809 to 0.301. The 21 accessions clustered into two major groups. Three exotic Choix accessions clustered together. Three other Choix accessions, collected from Guangxi, China, clustered into a cohesive subgroup. Four wild types of Choix clustered into the same subgroup. These results indicated that the classification by RAPD data reflected the differences in geographic origins and evolution in Choix.
AB - Choix, a plant in the tribe Maydeae of the grass family, has been cultivated in Asia for several thousand years. It is a potential gene resource for improvement of other cereal crops because of its nutritional value and tolerance to stress. Genetic variation and relationships among 21 Choix lachryma-jobi L. accessions were characterized by random amplified polymorphic DNA (RAPD) markers. A total of 205 DNA fragments across all materials were amplified with 31 random primers, averaging 6.61 per primer. Among amplified fragments, 115 showed polymorphism averaging 3.71 per primer. Of amplified markers, 56.1% were polymorphic, indicating considerable variation at the DNA level among these accessions. Some fragments were accession-specific. Pair-wise genetic similarity (GS) among 21 accessions ranged from 0.809 to 0.301. The 21 accessions clustered into two major groups. Three exotic Choix accessions clustered together. Three other Choix accessions, collected from Guangxi, China, clustered into a cohesive subgroup. Four wild types of Choix clustered into the same subgroup. These results indicated that the classification by RAPD data reflected the differences in geographic origins and evolution in Choix.
KW - Choix
KW - Genetic variation
KW - RAPD markers
UR - http://www.scopus.com/inward/record.url?scp=0034994672&partnerID=8YFLogxK
U2 - 10.1023/A:1011282109563
DO - 10.1023/A:1011282109563
M3 - 文章
AN - SCOPUS:0034994672
SN - 0925-9864
VL - 48
SP - 189
EP - 194
JO - Genetic Resources and Crop Evolution
JF - Genetic Resources and Crop Evolution
IS - 2
ER -