Bioelectrochemical methane production from CO₂ by Methanosarcina barkeri via direct and H₂-mediated indirect electron transfer

TY - JOUR

T1 - Bioelectrochemical methane production from CO2 by Methanosarcina barkeri via direct and H2-mediated indirect electron transfer

AU - Bai, Yang

AU - Zhou, Lei

AU - Irfan, Muhammad

AU - Liang, Tian Tian

AU - Cheng, Lei

AU - Liu, Yi Fan

AU - Liu, Jin Feng

AU - Yang, Shi Zhong

AU - Sand, Wolfgang

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

N1 - Publisher Copyright: © 2020 Elsevier Ltd

PY - 2020/11/1

Y1 - 2020/11/1

N2 - Electromethanogenesis is a promising strategy for bioconversion of CO2 to CH4 via direct electron transfer (DET) and/or H2-mediated indirect electron transfer (IET). However, specific contribution ratios of DET or H2-mediated IET are unclear. Here, pathway preferences were firstly calculated in the “cage” type cathode colonized by Methanosarcina barkeri. The highest CH4 production rate of 4.4 μmol cm−2•day−1 was detected from 36 to 72 h at −1.2 V. The average ratios of H2-mediated IET were calculated as 59.73 ± 8.29%, 69.45 ± 20.75%, 69.55 ± 18.30% and 82.97 ± 16.13% at −0.6, −0.8, −1.0 and −1.2 V (vs SHE), respectively. While above −0.4 V, only the DET pathway was detected. The real time polymerase-chain reaction amplification at the transcriptional level of ccdA and frhB showed the similar trend for the pathway preference in CH4 production. Results provide specific ratios and preference of CH4 production via these two pathways, which may be used for parameter reference for industrial applications.

AB - Electromethanogenesis is a promising strategy for bioconversion of CO2 to CH4 via direct electron transfer (DET) and/or H2-mediated indirect electron transfer (IET). However, specific contribution ratios of DET or H2-mediated IET are unclear. Here, pathway preferences were firstly calculated in the “cage” type cathode colonized by Methanosarcina barkeri. The highest CH4 production rate of 4.4 μmol cm−2•day−1 was detected from 36 to 72 h at −1.2 V. The average ratios of H2-mediated IET were calculated as 59.73 ± 8.29%, 69.45 ± 20.75%, 69.55 ± 18.30% and 82.97 ± 16.13% at −0.6, −0.8, −1.0 and −1.2 V (vs SHE), respectively. While above −0.4 V, only the DET pathway was detected. The real time polymerase-chain reaction amplification at the transcriptional level of ccdA and frhB showed the similar trend for the pathway preference in CH4 production. Results provide specific ratios and preference of CH4 production via these two pathways, which may be used for parameter reference for industrial applications.

KW - CO bioconversion

KW - Electromethanogenesis

KW - Electron transfer pathway

KW - Pure methanogen

UR - http://www.scopus.com/inward/record.url?scp=85089244890&partnerID=8YFLogxK

U2 - 10.1016/j.energy.2020.118445

DO - 10.1016/j.energy.2020.118445

M3 - 文章

AN - SCOPUS:85089244890

SN - 0360-5442

VL - 210

JO - Energy

JF - Energy

M1 - 118445

ER -