Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate ester by Pseudomonas fluorescens B-1

TY - JOUR

T1 - Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate ester by Pseudomonas fluorescens B-1

AU - Xu, Xiang Rong

AU - Li, Hua Bin

AU - Gu, Ji Dong

N1 - Funding Information: This research was partially supported by ITS/276/00 from the Innovative Technology Commission of Hong Kong SAR, and industrial partnerships with Kou Hing Hong Scientific Supplies and Peako Engineering Co. We would like to thank the technical laboratory support of Jessie Lai.

PY - 2005/1

Y1 - 2005/1

N2 - Di-n-butyl phthalate ester (DBP) is known as an endocrine-disrupting chemical. A pure culture capable of using DBP as the sole source of carbon and energy from mangrove sediment was identified as Pseudomonas fluorescens B-1. Microbial degradation of DBP was studied in batch experiments for several environmental factors. The effect of initial DBP concentrations on the degradation was investigated between 2.5 and 10.0 mg l-1, and the results showed that the biodegradation process conformed to the first-order kinetic model. The pH value of the culture medium also played an important role in the biodegradation of DBP, the optimum pH being 7.0. The effects of temperature and oxygen availability on the kinetics of DBP biodegradation were also determined. Degradation of DBP by P. fluorescens B-1 was quantified by reversed-phase high-performance liquid chromatography after solid-phase extraction. Two metabolites of DBP degradation were identified as mono-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry. The results suggest that DBP can be degraded by indigenous microorganisms from the mangrove environment.

AB - Di-n-butyl phthalate ester (DBP) is known as an endocrine-disrupting chemical. A pure culture capable of using DBP as the sole source of carbon and energy from mangrove sediment was identified as Pseudomonas fluorescens B-1. Microbial degradation of DBP was studied in batch experiments for several environmental factors. The effect of initial DBP concentrations on the degradation was investigated between 2.5 and 10.0 mg l-1, and the results showed that the biodegradation process conformed to the first-order kinetic model. The pH value of the culture medium also played an important role in the biodegradation of DBP, the optimum pH being 7.0. The effects of temperature and oxygen availability on the kinetics of DBP biodegradation were also determined. Degradation of DBP by P. fluorescens B-1 was quantified by reversed-phase high-performance liquid chromatography after solid-phase extraction. Two metabolites of DBP degradation were identified as mono-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry. The results suggest that DBP can be degraded by indigenous microorganisms from the mangrove environment.

KW - Biodegradation

KW - Di-n-butyl phthalate

KW - Endocrine-disruptor

KW - Kinetics

KW - Metabolites

KW - Plasticizer

UR - http://www.scopus.com/inward/record.url?scp=12344286255&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2004.05.005

DO - 10.1016/j.ibiod.2004.05.005

M3 - 文章

AN - SCOPUS:12344286255

SN - 0964-8305

VL - 55

SP - 9

EP - 15

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

IS - 1

ER -