Biocompatible fluorescent polyamine-based cyclophosphazene hybrid nanospheres for targeted cell imaging

TY - JOUR

T1 - Biocompatible fluorescent polyamine-based cyclophosphazene hybrid nanospheres for targeted cell imaging

AU - Akram, Raheel

AU - Arshad, Anila

AU - Dar, Sami Ullah

AU - Basharat, Majid

AU - Liu, Wei

AU - Zhang, Shuangkun

AU - Wu, Zhanpeng

AU - Wu, Dezhen

N1 - Publisher Copyright: © 2019 John Wiley & Sons, Ltd.

PY - 2020/3/1

Y1 - 2020/3/1

N2 - Fluorescent nanoprobes are highly desirable toolkit for bioimaging applications. This study reports the first example for the synthesis of a nontoxic prototypical fluorescent organic compound 2-benzo[d]thiazol-2-yl)-3-(2-chloro-4-(dimethylamino)phenyl)acrylonitrile (BCA) and its entrapment into the poly[cyclotriphosphazene-co-polyethyleneimine] cross-linked (PCPEI) nanospheres named as BCA@PCPEI for targeted cell imaging application. The as-prepared BCA@PCPEI nanospheres were thoroughly characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), fourier transform infrared (FTIR), thermogravimetric analysis (TGA), and phosphorus-31 nuclear magnetic resonance (31P-NMR) analyses. The surface functional analysis of the nanospheres was performed by X-ray photoelectron spectroscopy (XPS), which proves that the content ratios of elements belong to the precursors concentrations. The as-prepared nanospheres displayed emission at 606 nm with bright orange fluorescence at any concentration. Moreover, the nanospheres were also less cytotoxic and maintained remarkable cell viability up to 100 μg/mL. Owing to the fluorescence with higher emission, this material has shown excellent cell imaging performance with better targeting ability to HeLa cells.

AB - Fluorescent nanoprobes are highly desirable toolkit for bioimaging applications. This study reports the first example for the synthesis of a nontoxic prototypical fluorescent organic compound 2-benzo[d]thiazol-2-yl)-3-(2-chloro-4-(dimethylamino)phenyl)acrylonitrile (BCA) and its entrapment into the poly[cyclotriphosphazene-co-polyethyleneimine] cross-linked (PCPEI) nanospheres named as BCA@PCPEI for targeted cell imaging application. The as-prepared BCA@PCPEI nanospheres were thoroughly characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), fourier transform infrared (FTIR), thermogravimetric analysis (TGA), and phosphorus-31 nuclear magnetic resonance (31P-NMR) analyses. The surface functional analysis of the nanospheres was performed by X-ray photoelectron spectroscopy (XPS), which proves that the content ratios of elements belong to the precursors concentrations. The as-prepared nanospheres displayed emission at 606 nm with bright orange fluorescence at any concentration. Moreover, the nanospheres were also less cytotoxic and maintained remarkable cell viability up to 100 μg/mL. Owing to the fluorescence with higher emission, this material has shown excellent cell imaging performance with better targeting ability to HeLa cells.

KW - BCA@PCPEI

KW - fluorescent material

KW - nanospheres

KW - targeted cell imaging

UR - http://www.scopus.com/inward/record.url?scp=85075022169&partnerID=8YFLogxK

U2 - 10.1002/pat.4778

DO - 10.1002/pat.4778

M3 - 文章

AN - SCOPUS:85075022169

SN - 1042-7147

VL - 31

SP - 425

EP - 432

JO - Polymers for Advanced Technologies

JF - Polymers for Advanced Technologies

IS - 3

ER -