TY - JOUR
T1 - Biochemical composite material using corncob powder as a carrier material for ureolytic bacteria in soil cadmium immobilization
AU - Li, Weila
AU - Yang, Yifan
AU - Achal, Varenyam
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - Corncob powder possessing its superiority in environmental sustainability and cost, was approved with strong capability of being a replacement of biochar in facilitating the microbial carbonate precipitation process. In this study, the ureolytic bacterial strain Bacillus sp. WA isolated from a pre-acquired metal contaminated soil in Guiyu, China, was showed to be well attached on the surfaces of corncob powder, indicating the carrier's role as a durable shelter for bacterial cells. The efficient immobilization helped develop biochemical composite material (BCM) and proven to function better the calcite precipitation. Afterwards, the mechanism and multi-directional benefits of BCM in edaphic cadmium remediation were examined through pot experiment and compared with corncob powder/bacterial strain/nutrient media as control groups. Integrated lab-scale analyses emphasized the advantages of BCM by the maximum soil urease activity (up to 3.440 U/mg and increased by 214% in 28 days), maximal bacterial propagation (most abundant population in fluorescence microscopy), richest surface functional group (most remarkable O[sbnd]C bond and C[dbnd]O bond in FTIR result), notable calcite precipitation (clear calcite crystals on the surface of BCM compared to control group under SEM-EDS), and highest Cd immobilization rate (exchangeable Cd decreased by 68.54%), among all treatments. The pH and electroconductivity measurements additionally led to the mechanism of corncob powder and NBU promoting pre-existed ureolytic bacteria in soil, which demonstrated the added value of corncob to be fine carbon source and residence shelter for soil microorganism, revealing its potential in developing agricultural materials.
AB - Corncob powder possessing its superiority in environmental sustainability and cost, was approved with strong capability of being a replacement of biochar in facilitating the microbial carbonate precipitation process. In this study, the ureolytic bacterial strain Bacillus sp. WA isolated from a pre-acquired metal contaminated soil in Guiyu, China, was showed to be well attached on the surfaces of corncob powder, indicating the carrier's role as a durable shelter for bacterial cells. The efficient immobilization helped develop biochemical composite material (BCM) and proven to function better the calcite precipitation. Afterwards, the mechanism and multi-directional benefits of BCM in edaphic cadmium remediation were examined through pot experiment and compared with corncob powder/bacterial strain/nutrient media as control groups. Integrated lab-scale analyses emphasized the advantages of BCM by the maximum soil urease activity (up to 3.440 U/mg and increased by 214% in 28 days), maximal bacterial propagation (most abundant population in fluorescence microscopy), richest surface functional group (most remarkable O[sbnd]C bond and C[dbnd]O bond in FTIR result), notable calcite precipitation (clear calcite crystals on the surface of BCM compared to control group under SEM-EDS), and highest Cd immobilization rate (exchangeable Cd decreased by 68.54%), among all treatments. The pH and electroconductivity measurements additionally led to the mechanism of corncob powder and NBU promoting pre-existed ureolytic bacteria in soil, which demonstrated the added value of corncob to be fine carbon source and residence shelter for soil microorganism, revealing its potential in developing agricultural materials.
KW - Bioremediation
KW - Cadmium
KW - Corncob
KW - MICP
KW - Ureolytic bacteria
UR - http://www.scopus.com/inward/record.url?scp=85113731361&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2021.149802
DO - 10.1016/j.scitotenv.2021.149802
M3 - 文章
C2 - 34464799
AN - SCOPUS:85113731361
SN - 0048-9697
VL - 802
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 149802
ER -