Azo dye degrading bacteria tolerant to extreme conditions inhabit nearshore ecosystems: Optimization and degradation pathways

Mei Zhuang; Edmond Sanganyado; Xinxin Zhang; Liang Xu; Jianming Zhu; Wenhua Liu; Haihong Song

doi:10.1016/j.jenvman.2020.110222

Azo dye degrading bacteria tolerant to extreme conditions inhabit nearshore ecosystems: Optimization and degradation pathways

Mei Zhuang, Edmond Sanganyado, Xinxin Zhang, Liang Xu, Jianming Zhu, Wenhua Liu^*, Haihong Song

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Nearshore ecosystems are transitional zones, and they may harbor a diverse microbial community capable of degrading azo dyes under extreme environmental conditions. In this study, thirteen bacterial strains capable of degrading eight azo dyes were isolated in nearshore environments and characterized using high throughput 16 S rRNA sequencing. The results of this study demonstrate that the biodegradability of azo dyes was influenced by their chemical structure and position of functional groups as well as the type of bacteria. The decolorization rate of Methyl Orange (95%) was double that of the heavier and sterically hindered Reactive Yellow 84 (<40%). Shewanella indica strain ST2, Oceanimonas smirnovii strain ST3, Enterococcus faecalis strain ST5, and Clostridium bufermentans strain ST12 demonstrated potential application in industrial effluent treatment as they were tolerant to a wide range of environmental parameters (pH: 5–9, NaCl: 0–70 g L⁻¹, azo dye concentration: 100–2000 mg L⁻¹) including exposure to metals. Analysis of the transformation products using GC-MS revealed that different bacterial strains may have different biotransformation pathways. This study provides critical insight on the in-situ biotransformation potential of azo dyes in marine environments.

Original language	English
Article number	110222
Journal	Journal of Environmental Management
Volume	261
DOIs	https://doi.org/10.1016/j.jenvman.2020.110222
State	Published - 1 May 2020
Externally published	Yes

Keywords

Azo dye-degrading bacteria
Azo reductase
Biodegradation
High throughput sequencing
Nearshore sediment

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jenvman.2020.110222

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title = "Azo dye degrading bacteria tolerant to extreme conditions inhabit nearshore ecosystems: Optimization and degradation pathways",

abstract = "Nearshore ecosystems are transitional zones, and they may harbor a diverse microbial community capable of degrading azo dyes under extreme environmental conditions. In this study, thirteen bacterial strains capable of degrading eight azo dyes were isolated in nearshore environments and characterized using high throughput 16 S rRNA sequencing. The results of this study demonstrate that the biodegradability of azo dyes was influenced by their chemical structure and position of functional groups as well as the type of bacteria. The decolorization rate of Methyl Orange (95%) was double that of the heavier and sterically hindered Reactive Yellow 84 (<40%). Shewanella indica strain ST2, Oceanimonas smirnovii strain ST3, Enterococcus faecalis strain ST5, and Clostridium bufermentans strain ST12 demonstrated potential application in industrial effluent treatment as they were tolerant to a wide range of environmental parameters (pH: 5–9, NaCl: 0–70 g L−1, azo dye concentration: 100–2000 mg L−1) including exposure to metals. Analysis of the transformation products using GC-MS revealed that different bacterial strains may have different biotransformation pathways. This study provides critical insight on the in-situ biotransformation potential of azo dyes in marine environments.",

keywords = "Azo dye-degrading bacteria, Azo reductase, Biodegradation, High throughput sequencing, Nearshore sediment",

author = "Mei Zhuang and Edmond Sanganyado and Xinxin Zhang and Liang Xu and Jianming Zhu and Wenhua Liu and Haihong Song",

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AU - Zhuang, Mei

AU - Sanganyado, Edmond

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AU - Xu, Liang

AU - Zhu, Jianming

AU - Liu, Wenhua

AU - Song, Haihong

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N2 - Nearshore ecosystems are transitional zones, and they may harbor a diverse microbial community capable of degrading azo dyes under extreme environmental conditions. In this study, thirteen bacterial strains capable of degrading eight azo dyes were isolated in nearshore environments and characterized using high throughput 16 S rRNA sequencing. The results of this study demonstrate that the biodegradability of azo dyes was influenced by their chemical structure and position of functional groups as well as the type of bacteria. The decolorization rate of Methyl Orange (95%) was double that of the heavier and sterically hindered Reactive Yellow 84 (<40%). Shewanella indica strain ST2, Oceanimonas smirnovii strain ST3, Enterococcus faecalis strain ST5, and Clostridium bufermentans strain ST12 demonstrated potential application in industrial effluent treatment as they were tolerant to a wide range of environmental parameters (pH: 5–9, NaCl: 0–70 g L−1, azo dye concentration: 100–2000 mg L−1) including exposure to metals. Analysis of the transformation products using GC-MS revealed that different bacterial strains may have different biotransformation pathways. This study provides critical insight on the in-situ biotransformation potential of azo dyes in marine environments.

AB - Nearshore ecosystems are transitional zones, and they may harbor a diverse microbial community capable of degrading azo dyes under extreme environmental conditions. In this study, thirteen bacterial strains capable of degrading eight azo dyes were isolated in nearshore environments and characterized using high throughput 16 S rRNA sequencing. The results of this study demonstrate that the biodegradability of azo dyes was influenced by their chemical structure and position of functional groups as well as the type of bacteria. The decolorization rate of Methyl Orange (95%) was double that of the heavier and sterically hindered Reactive Yellow 84 (<40%). Shewanella indica strain ST2, Oceanimonas smirnovii strain ST3, Enterococcus faecalis strain ST5, and Clostridium bufermentans strain ST12 demonstrated potential application in industrial effluent treatment as they were tolerant to a wide range of environmental parameters (pH: 5–9, NaCl: 0–70 g L−1, azo dye concentration: 100–2000 mg L−1) including exposure to metals. Analysis of the transformation products using GC-MS revealed that different bacterial strains may have different biotransformation pathways. This study provides critical insight on the in-situ biotransformation potential of azo dyes in marine environments.

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KW - Azo reductase

KW - Biodegradation

KW - High throughput sequencing

KW - Nearshore sediment

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Azo dye degrading bacteria tolerant to extreme conditions inhabit nearshore ecosystems: Optimization and degradation pathways

Abstract

Keywords

UN SDGs

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