TY - JOUR
T1 - Association mapping of starch physicochemical properties with starch synthesis-related gene markers in nonwaxy rice (Oryza sativa L.)
AU - Yang, Feng
AU - Chen, Yaling
AU - Tong, Chuan
AU - Huang, Yan
AU - Xu, Feifei
AU - Li, Kehu
AU - Corke, Harold
AU - Sun, Mei
AU - Bao, Jinsong
N1 - Publisher Copyright:
© 2014, Springer Science+Business Media Dordrecht.
PY - 2014/12/6
Y1 - 2014/12/6
N2 - Starch physicochemical properties strongly influence eating and cooking quality of rice. The cleaved amplified polymorphic sequences, derived CAPS, and InDel markers for 13 starch synthesis-related genes (SSRGs) were developed, and together with markers developed before, there are 35 markers tagged for 23 SSRGs, with each gene tagged with at least one marker. These and 108 other markers were used for association mapping for 20 starch physicochemical property parameters. A total of 64 main-effect loci or quantitative trait locus (QTLs) was detected. In addition, 56 and 62 loci were identified under the Wx and SSIIa background, respectively. Wx was a major main-effect QTL for apparent amylose content (AAC), pasting viscosity, gel texture, and retrogradation property (P < 0.0001). SSIIa was a major main-effect QTL for pasting temperature, thermal, and retrogradation properties (P < 0.0001), but it was a minor main-effect QTL for some pasting viscosity parameters, such as BD, CS, Stab, and SBratio. Four other SSRGs, SSIIa, BE1, SSIIc, and GBSSII were detected for AAC under Wx background. Wx was detected for Tc and ΔHg under the SSIIa background. PUL was detected for HD as main-effect QTL and under SSIIa background. AGPL2 and ISA1 were detected, respectively, for ΔHg and retrogradation as main-effect QTL, as well as under both Wx and SSIIa backgrounds. This study suggested that retrogradation properties were mainly controlled by Wx, SSIIa, and ISA1 with the relative effects in the order of SSIIa > Wx > ISA1. These results have direct applications to quality breeding programs.
AB - Starch physicochemical properties strongly influence eating and cooking quality of rice. The cleaved amplified polymorphic sequences, derived CAPS, and InDel markers for 13 starch synthesis-related genes (SSRGs) were developed, and together with markers developed before, there are 35 markers tagged for 23 SSRGs, with each gene tagged with at least one marker. These and 108 other markers were used for association mapping for 20 starch physicochemical property parameters. A total of 64 main-effect loci or quantitative trait locus (QTLs) was detected. In addition, 56 and 62 loci were identified under the Wx and SSIIa background, respectively. Wx was a major main-effect QTL for apparent amylose content (AAC), pasting viscosity, gel texture, and retrogradation property (P < 0.0001). SSIIa was a major main-effect QTL for pasting temperature, thermal, and retrogradation properties (P < 0.0001), but it was a minor main-effect QTL for some pasting viscosity parameters, such as BD, CS, Stab, and SBratio. Four other SSRGs, SSIIa, BE1, SSIIc, and GBSSII were detected for AAC under Wx background. Wx was detected for Tc and ΔHg under the SSIIa background. PUL was detected for HD as main-effect QTL and under SSIIa background. AGPL2 and ISA1 were detected, respectively, for ΔHg and retrogradation as main-effect QTL, as well as under both Wx and SSIIa backgrounds. This study suggested that retrogradation properties were mainly controlled by Wx, SSIIa, and ISA1 with the relative effects in the order of SSIIa > Wx > ISA1. These results have direct applications to quality breeding programs.
KW - Amylose
KW - Association mapping
KW - Eating quality
KW - Gelatinization temperature
KW - QTL
KW - RVA pasting viscosity
KW - Rice
UR - http://www.scopus.com/inward/record.url?scp=84914812830&partnerID=8YFLogxK
U2 - 10.1007/s11032-014-0135-y
DO - 10.1007/s11032-014-0135-y
M3 - 文章
AN - SCOPUS:84914812830
SN - 1380-3743
VL - 34
SP - 1747
EP - 1763
JO - Molecular Breeding
JF - Molecular Breeding
IS - 4
ER -