Architecture of outer shell and inner blocklets of rice starch granule is related to starch granule-associated proteins

Mengting Ma, Zekun Xu, Xiaojing Chen, Chuangchuang Zhang, Ziyi Liu, Dennis Cantre, Haitao Li*, Zhongquan Sui*, Harold Corke

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Waxy, low-amylose and high-amylose rice starches were chosen to separate inner blocklets and outer shell from their granules under incomplete gelatinization. Isolated outer shells and swollen or merged inner blocklets, based on morphological structure of broken granules, were used as a probe to study the mechanism of differences between outer and inner blocklets formation, through molecular characteristics and starch granule-associated proteins (SGAPs) analyses. On the basis of molecular composition, it was concluded that shorter branched-chain amylopectin and some amylose chains were involved in the formation of inner blocklets (shells). While the stronger interaction occurred among outer blocklets (shell), those are composed of longer branched-chains amylopectin and few amylose molecules. SGAPs types and content of inner shells (blocklets) were higher than outer shell (blocklets). SGAPs not only interacted with amylose chains but also with amylopectin chains. The enzymatic machinery for biosynthesis, such as starch branching enzyme, plays a critical role in blocklet architecture.
Original languageEnglish
JournalFood Hydrocolloids
DOIs
StatePublished - 1 Jun 2022

Keywords

  • Rice starch
  • Outer shell
  • Inner blocklets
  • Amylopectin
  • Starch granule-associated proteins
  • Starch synthesis enzymes

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