TY - JOUR
T1 - Abundance of ammonia-oxidizing bacteria and archaea under different ventilation strategies during cattle manure composting
AU - Yan, Lei
AU - Wang, Guoxing
AU - Ai, Shiqi
AU - Huo, Zhaochen
AU - Wang, Yanjie
AU - Gu, Ji Dong
AU - Wang, Weidong
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/4/15
Y1 - 2018/4/15
N2 - Composting of cattle manure was conducted under four ventilation strategies, i.e., no-aeration (A-00), continuous aeration (B-44), non-aeration for 14 d and then aeration for 42 d (C-04), aeration for 14 d and then no-aeration for 42 d (D-40). Physicochemical parameters and potential ammonia oxidation (PAO) indicated that continuous and intermittent ventilation provide favourable conditions for ammonia-oxidizing bacteria (AOB) and archaea (AOA) to oxidize ammonia. Quantitative PCR (qPCR) analysis showed AOB amoA gene abundance of all treatments on every sampling day ranged from 2.25 × 105 to 2.76 × 109copies/g, was significantly lower than that of archaeal amoA gene from 2.71 × 108 to 9.05 × 1011copies/g. There was also a significantly positive relationship between PAO rates and AOB (r2 ≥ 0.066, p < 0.05) and AOA (r2 ≥ 0.300, p < 0.05) abundance. These data suggested that ammonia oxidation is driven by both AOA and AOB in cattle manure composting.
AB - Composting of cattle manure was conducted under four ventilation strategies, i.e., no-aeration (A-00), continuous aeration (B-44), non-aeration for 14 d and then aeration for 42 d (C-04), aeration for 14 d and then no-aeration for 42 d (D-40). Physicochemical parameters and potential ammonia oxidation (PAO) indicated that continuous and intermittent ventilation provide favourable conditions for ammonia-oxidizing bacteria (AOB) and archaea (AOA) to oxidize ammonia. Quantitative PCR (qPCR) analysis showed AOB amoA gene abundance of all treatments on every sampling day ranged from 2.25 × 105 to 2.76 × 109copies/g, was significantly lower than that of archaeal amoA gene from 2.71 × 108 to 9.05 × 1011copies/g. There was also a significantly positive relationship between PAO rates and AOB (r2 ≥ 0.066, p < 0.05) and AOA (r2 ≥ 0.300, p < 0.05) abundance. These data suggested that ammonia oxidation is driven by both AOA and AOB in cattle manure composting.
KW - Ammonia-oxidizing microorganisms
KW - Cattle manure composting
KW - Potential ammonia oxidation
KW - Ventilation strategy
KW - amoA gene abundance
UR - http://www.scopus.com/inward/record.url?scp=85042172861&partnerID=8YFLogxK
U2 - 10.1016/j.jenvman.2018.02.032
DO - 10.1016/j.jenvman.2018.02.032
M3 - 文章
C2 - 29459337
AN - SCOPUS:85042172861
SN - 0301-4797
VL - 212
SP - 375
EP - 383
JO - Journal of Environmental Management
JF - Journal of Environmental Management
ER -