A facile and efficient pretreatment of corncob for bioproduction of butanol

Wei Luo*, Jie Wang, Xb Liu, Hanguang Li, Hepeng Pan, Qiuya Gu, Xiaobin Yu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


The present study developed a combined ball milling-aqueous swelling (CBMAS) pretreatment to accelerate the hydrolysis of corncob. The enzymatic hydrolysis of microcrystalline cellulose carried out in the plates and flasks indicated that the response of enzymatic hydrolysis to CBMAS was quite evident. The fermentable reducing sugars of hydrolysates from CBMAS-pretreated corncob was 59.8. g/L, which was 1.3 and 1.7 folds higher than those from diluted acid and alkaline pretreated corncob hydrolysates, respectively. Simultaneous CBMAS pretreatment and enzymatic hydrolysis was also conducted, reducing the processing time from 66. h to 28. h. The enzymatic hydrolysates from CBMAS-pretreated corncob could be directly utilized as the substrate for butanol fermentation without detoxication. Under the optimal conditions, fermentable sugars in the corncob hydrolysate were completely consumed to generate 9.52. g/L butanol.

Original languageEnglish
Pages (from-to)86-89
Number of pages4
JournalBioresource Technology
StatePublished - Jul 2013
Externally publishedYes


  • Butanol fermentation
  • Combined ball milling-aqueous swelling
  • Corncob
  • Pretreatment


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