A facile and efficient pretreatment of corncob for bioproduction of butanol

TY - JOUR

T1 - A facile and efficient pretreatment of corncob for bioproduction of butanol

AU - Luo, Wei

AU - Wang, Jie

AU - Liu, Xb

AU - Li, Hanguang

AU - Pan, Hepeng

AU - Gu, Qiuya

AU - Yu, Xiaobin

N1 - Funding Information: This work was supported by National Natural Science Foundation of China (Grant No. 21176105 ), the Fundamental Research Funds for the Central Universities (Grant No. JUSRP111A24 ), Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (Grant No. KLIB-KF201105 ), the 111 Project (No. 111-2-06 ) and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions .

PY - 2013/7

Y1 - 2013/7

N2 - The present study developed a combined ball milling-aqueous swelling (CBMAS) pretreatment to accelerate the hydrolysis of corncob. The enzymatic hydrolysis of microcrystalline cellulose carried out in the plates and flasks indicated that the response of enzymatic hydrolysis to CBMAS was quite evident. The fermentable reducing sugars of hydrolysates from CBMAS-pretreated corncob was 59.8. g/L, which was 1.3 and 1.7 folds higher than those from diluted acid and alkaline pretreated corncob hydrolysates, respectively. Simultaneous CBMAS pretreatment and enzymatic hydrolysis was also conducted, reducing the processing time from 66. h to 28. h. The enzymatic hydrolysates from CBMAS-pretreated corncob could be directly utilized as the substrate for butanol fermentation without detoxication. Under the optimal conditions, fermentable sugars in the corncob hydrolysate were completely consumed to generate 9.52. g/L butanol.

AB - The present study developed a combined ball milling-aqueous swelling (CBMAS) pretreatment to accelerate the hydrolysis of corncob. The enzymatic hydrolysis of microcrystalline cellulose carried out in the plates and flasks indicated that the response of enzymatic hydrolysis to CBMAS was quite evident. The fermentable reducing sugars of hydrolysates from CBMAS-pretreated corncob was 59.8. g/L, which was 1.3 and 1.7 folds higher than those from diluted acid and alkaline pretreated corncob hydrolysates, respectively. Simultaneous CBMAS pretreatment and enzymatic hydrolysis was also conducted, reducing the processing time from 66. h to 28. h. The enzymatic hydrolysates from CBMAS-pretreated corncob could be directly utilized as the substrate for butanol fermentation without detoxication. Under the optimal conditions, fermentable sugars in the corncob hydrolysate were completely consumed to generate 9.52. g/L butanol.

KW - Butanol fermentation

KW - Combined ball milling-aqueous swelling

KW - Corncob

KW - Pretreatment

UR - http://www.scopus.com/inward/record.url?scp=84877914544&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2013.04.063

DO - 10.1016/j.biortech.2013.04.063

M3 - 文章

C2 - 23681001

AN - SCOPUS:84877914544

SN - 0960-8524

VL - 140

SP - 86

EP - 89

JO - Bioresource Technology

JF - Bioresource Technology

ER -