TY - JOUR
T1 - Two identical copies of the hydrazine synthase gene clusters found in the genomes of anammox bacteria
AU - Yang, Yuchun
AU - Li, Meng
AU - Li, Xiao Yan
AU - Gu, Ji Dong
N1 - Publisher Copyright:
© 2018
PY - 2018/8
Y1 - 2018/8
N2 - Hydrazine synthase (HZS), encoded by the hzsABC gene cluster, is the unique and irreplaceable enzyme for the anammox reaction in this group of bacteria. This gene is widely used as a functional gene biomarker to retrieve the diversity, abundance and activity of anammox bacteria in different ecological niches. Genomes of anammox bacteria are constantly acquired, but the copy number of hzsABC gene cluster in the genome of anammox bacteria remains unsettled. In this study, three high quality anammox bacterial genomes were acquired from metagenomic datasets of activated sludge samples containing anammox bacteria. Interestingly, the coverage of hzsABC gene cluster in all of the three genomes was approximately two folds, comparing to the coverage of the corresponding genomic bin. However, only one assembled scaffold contained the full length of hzsABC in each genome, therefore it is likely that two identical copies of the hzsABC gene cluster are present in each of the three genomes. To confirm this, specific PCR primers were designed based on the assembled scaffolds containing the full-length hzsABC and partial hzsC genes. Two complete sequences connecting with the 5’ terminal of hzsC were successfully amplified using genomic DNA as templates, which further supported our hypothesis of the two identical copies of the gene. Moreover, the twofold coverage relationships of hzs genes and genomes were also found in the published genomes of“Ca. Brocadia sinica” “Ca. Brocadia_caroliniensis” “Ca. Brocadia_fulgida”. Overall, we would conclude that the genome of anammox bacteria contains two identical copies of hzsABC gene cluster, but functionality of the two identical genes needs further confirmations.
AB - Hydrazine synthase (HZS), encoded by the hzsABC gene cluster, is the unique and irreplaceable enzyme for the anammox reaction in this group of bacteria. This gene is widely used as a functional gene biomarker to retrieve the diversity, abundance and activity of anammox bacteria in different ecological niches. Genomes of anammox bacteria are constantly acquired, but the copy number of hzsABC gene cluster in the genome of anammox bacteria remains unsettled. In this study, three high quality anammox bacterial genomes were acquired from metagenomic datasets of activated sludge samples containing anammox bacteria. Interestingly, the coverage of hzsABC gene cluster in all of the three genomes was approximately two folds, comparing to the coverage of the corresponding genomic bin. However, only one assembled scaffold contained the full length of hzsABC in each genome, therefore it is likely that two identical copies of the hzsABC gene cluster are present in each of the three genomes. To confirm this, specific PCR primers were designed based on the assembled scaffolds containing the full-length hzsABC and partial hzsC genes. Two complete sequences connecting with the 5’ terminal of hzsC were successfully amplified using genomic DNA as templates, which further supported our hypothesis of the two identical copies of the gene. Moreover, the twofold coverage relationships of hzs genes and genomes were also found in the published genomes of“Ca. Brocadia sinica” “Ca. Brocadia_caroliniensis” “Ca. Brocadia_fulgida”. Overall, we would conclude that the genome of anammox bacteria contains two identical copies of hzsABC gene cluster, but functionality of the two identical genes needs further confirmations.
KW - Anammox bacteria
KW - Genome
KW - Hydrazine synthase
KW - Nitrogen cycle
KW - Wastewater treatment
KW - hzs gene
UR - http://www.scopus.com/inward/record.url?scp=85046781739&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2018.04.011
DO - 10.1016/j.ibiod.2018.04.011
M3 - 文章
AN - SCOPUS:85046781739
SN - 0964-8305
VL - 132
SP - 236
EP - 240
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
ER -