The diversity of hydrogen-producing microorganisms in a high temperature oil reservoir and its potential role in promoting the in situ bioprocess

Jin Feng Liu, Serge Maurice Mbadinga, Wen Ji Ke, Ji Dong Gu, Bo Zhong Mu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Hydrogen-producing microorganisms are believed to play an important role in energy metabolism of microorganisms in anaerobic environments and hence are one of the crucial factors for influencing the activity and development of these microorganisms. Consequently, they provide the biological foundation for the biotechnology such as MEOR (Microbial Enhanced Oil Recovery) and microbial fixation of CO2 and conversion of it into CH4 and etc. How-ever, knowledge on the community of hydrogen-producing microorganisms and their potential in subsurface formations are still limited. In this study, hydrogen-producing microorganisms in the production water from an oilfield as well as enrichment cultures were analyzed with clone library analysis of [FeFe]-hydrogenase encoding genes. The results show that [FeFe]-hydrogenase genes in production water are diverse and related to Bacteroidetes, Firmicutes, Spirochaetes and uncultured. Anaerobic incubations established within the oil reservoir production water and generating 202 mmol H2/mol glucose during 7-day incubation at 55°C indicate a high frequency of members of the Firmicutes. This study implies that hydrogen-producing microorganisms in oil reservoir may play a positive role in promoting the in situ bio-process via hydrogen production once common nutrients are available. These data are helpful for evaluating, developing, and utilizing hydrogen-producing microorganisms in oil reservoirs for biological fixation and conversion of CO2 into CH4 as well as MEOR.

Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalApplied Environmental Biotechnology
Volume2
Issue number1
DOIs
StatePublished - 2017
Externally publishedYes

Keywords

  • Enrichment culture
  • Functional gene biomarker
  • Hydrogen-producing microorganisms
  • Microbial community
  • Oil reservoir

Fingerprint Dive into the research topics of 'The diversity of hydrogen-producing microorganisms in a high temperature oil reservoir and its potential role in promoting the in situ bioprocess'. Together they form a unique fingerprint.

Cite this