Structures of building blocks in clusters of sweetpotato amylopectin

Fan Zhu*, Harold Corke, Per Man, Eric Bertoft

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

,β-Limit dextrins of domains and clusters of sweetpotato amylopectin were subjected to extensive hydrolysis by Bacillus amyloliquefaciens α-amylase to release building blocks and reveal the internal structures of clusters. The composition of building blocks was analyzed by size-fractionation, gel permeation chromatography, and high performance anion exchange chromatography. Different domains and clusters had structurally similar building blocks with around three chains per building block and internal chain length around 2.9. Singly branched and doubly branched building blocks were the largest and second largest groups in the clusters. Type A clusters had more large building blocks and contained 5-6 blocks per cluster with an inter-block chain length (IB-CL) of 7.0, whereas type B clusters had less large building blocks and contained 3-4 blocks per cluster with IB-CL 7.9. Models on how the building blocks could be organized into type A and type B clusters are discussed.

Original languageEnglish
Pages (from-to)2913-2925
Number of pages13
JournalCarbohydrate Research
Volume346
Issue number18
DOIs
StatePublished - 27 Dec 2011
Externally publishedYes

Keywords

  • Bacillus amyloliquefaciens α-amylase
  • Building block
  • C-Type starch
  • Cluster
  • Sweetpotato amylopectin structure

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