Structures of building blocks in clusters of sweetpotato amylopectin

TY - JOUR

T1 - Structures of building blocks in clusters of sweetpotato amylopectin

AU - Zhu, Fan

AU - Corke, Harold

AU - Man, Per

AU - Bertoft, Eric

N1 - Funding Information: Kind advice from Dr. Kristine Koch and Dr. Roger Andersson of the Department of Food Science, Swedish University of Agricultural Sciences, Uppsala, Sweden, is gratefully acknowledged. This research was supported by The University of Hong Kong Seed Funding for Basic Research and The University of Hong Kong Committee on Research and Conference Grants.

PY - 2011/12/27

Y1 - 2011/12/27

N2 - ,β-Limit dextrins of domains and clusters of sweetpotato amylopectin were subjected to extensive hydrolysis by Bacillus amyloliquefaciens α-amylase to release building blocks and reveal the internal structures of clusters. The composition of building blocks was analyzed by size-fractionation, gel permeation chromatography, and high performance anion exchange chromatography. Different domains and clusters had structurally similar building blocks with around three chains per building block and internal chain length around 2.9. Singly branched and doubly branched building blocks were the largest and second largest groups in the clusters. Type A clusters had more large building blocks and contained 5-6 blocks per cluster with an inter-block chain length (IB-CL) of 7.0, whereas type B clusters had less large building blocks and contained 3-4 blocks per cluster with IB-CL 7.9. Models on how the building blocks could be organized into type A and type B clusters are discussed.

AB - ,β-Limit dextrins of domains and clusters of sweetpotato amylopectin were subjected to extensive hydrolysis by Bacillus amyloliquefaciens α-amylase to release building blocks and reveal the internal structures of clusters. The composition of building blocks was analyzed by size-fractionation, gel permeation chromatography, and high performance anion exchange chromatography. Different domains and clusters had structurally similar building blocks with around three chains per building block and internal chain length around 2.9. Singly branched and doubly branched building blocks were the largest and second largest groups in the clusters. Type A clusters had more large building blocks and contained 5-6 blocks per cluster with an inter-block chain length (IB-CL) of 7.0, whereas type B clusters had less large building blocks and contained 3-4 blocks per cluster with IB-CL 7.9. Models on how the building blocks could be organized into type A and type B clusters are discussed.

KW - Bacillus amyloliquefaciens α-amylase

KW - Building block

KW - C-Type starch

KW - Cluster

KW - Sweetpotato amylopectin structure

UR - http://www.scopus.com/inward/record.url?scp=82155168229&partnerID=8YFLogxK

U2 - 10.1016/j.carres.2011.10.009

DO - 10.1016/j.carres.2011.10.009

M3 - 文章

C2 - 22070830

AN - SCOPUS:82155168229

SN - 0008-6215

VL - 346

SP - 2913

EP - 2925

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 18

ER -