TY - JOUR
T1 - Structural basis for the secretion of evpc
T2 - A key type vi secretion system protein from edwardsiella tarda
AU - Jobichen, Chacko
AU - Chakraborty, Smarajit
AU - Li, Mo
AU - Zheng, Jun
AU - Joseph, Lissa
AU - Mok, Yu Keung
AU - Leung, Yin Kin
AU - Sivaraman, J.
PY - 2010
Y1 - 2010
N2 - The recently identified type VI secretion system (T6SS) is implicated in the virulence of many Gram-negative bacteria.Edwardsiella tarda is an important cause of hemorrhagic septicemia in fish and also gastro- and extra-intestinal infections inhumans. The E. tarda virulent protein (EVP) gene cluster encodes a conserved T6SS which contains 16 open reading frames.EvpC is one of the three major EVP secreted proteins and shares high sequence similarity with Hcp1, a key T6SS virulencefactor from Pseudomonas aeruginosa. EvpC contributes to the virulence of E. tarda by playing an essential role in functionalT6SS. Here, we report the crystal structure of EvpC from E. tarda PPD130/91 at a 2.8 A°resolution, along with functionalstudies of the protein. EvpC has a b-barrel domain with extended loops. The b-barrel consists of 11 anti-parallel b-strandswith an a-helix located on one side. In solution, EvpC exists as a dimer at low concentration and as a hexamer at higherconcentration. In the crystal, the symmetry related EvpC molecules form hexameric rings which stack together to form atube similar to Hcp1. Structure based mutagenesis revealed that N-terminal negatively charged residues, Asp4, Glu15 andGlu26, and C-terminal positively charged residues, Lys161, Lys162 and Lys163, played crucial roles in the secretion of EvpC.Moreover, the localization study indicates the presence of wild type EvpC in cytoplasm, periplasm and secreted fractions,whereas the N-terminal and C-terminal mutants were found mostly in the periplasmic region and was completely absent inthe secreted fraction. Results reported here provide insight into the structure, assembly and function of EvpC. Further, thesefindings can be extended to other EvpC homologs for understanding the mechanism of T6SS and targeting T6SS mediatedvirulence in Gram-negative pathogens.
AB - The recently identified type VI secretion system (T6SS) is implicated in the virulence of many Gram-negative bacteria.Edwardsiella tarda is an important cause of hemorrhagic septicemia in fish and also gastro- and extra-intestinal infections inhumans. The E. tarda virulent protein (EVP) gene cluster encodes a conserved T6SS which contains 16 open reading frames.EvpC is one of the three major EVP secreted proteins and shares high sequence similarity with Hcp1, a key T6SS virulencefactor from Pseudomonas aeruginosa. EvpC contributes to the virulence of E. tarda by playing an essential role in functionalT6SS. Here, we report the crystal structure of EvpC from E. tarda PPD130/91 at a 2.8 A°resolution, along with functionalstudies of the protein. EvpC has a b-barrel domain with extended loops. The b-barrel consists of 11 anti-parallel b-strandswith an a-helix located on one side. In solution, EvpC exists as a dimer at low concentration and as a hexamer at higherconcentration. In the crystal, the symmetry related EvpC molecules form hexameric rings which stack together to form atube similar to Hcp1. Structure based mutagenesis revealed that N-terminal negatively charged residues, Asp4, Glu15 andGlu26, and C-terminal positively charged residues, Lys161, Lys162 and Lys163, played crucial roles in the secretion of EvpC.Moreover, the localization study indicates the presence of wild type EvpC in cytoplasm, periplasm and secreted fractions,whereas the N-terminal and C-terminal mutants were found mostly in the periplasmic region and was completely absent inthe secreted fraction. Results reported here provide insight into the structure, assembly and function of EvpC. Further, thesefindings can be extended to other EvpC homologs for understanding the mechanism of T6SS and targeting T6SS mediatedvirulence in Gram-negative pathogens.
UR - http://www.scopus.com/inward/record.url?scp=77958517967&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0012910
DO - 10.1371/journal.pone.0012910
M3 - 文章
C2 - 20886112
AN - SCOPUS:77958517967
SN - 1932-6203
VL - 5
JO - PLoS ONE
JF - PLoS ONE
IS - 9
M1 - e12910
ER -