TY - JOUR
T1 - Spatial distribution and abundances of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in mangrove sediments
AU - Li, Meng
AU - Cao, Huiluo
AU - Hong, Yiguo
AU - Gu, Ji Dong
N1 - Funding Information:
Acknowledgements This research was supported in part by grants from Agriculture, Fisheries, and Conservation Department of the Hong Kong SAR Government, and Faculty of Science on Sustainable Water at The University of Hong Kong (to J-DG); National Natural Science Foundation of China (3080032), Knowledge Innovation Key Project of The Chinese Academy of Sciences (KZCX2-YW-QN207), Guangdong Province Natural Science Foundation (84510301001692), and a start-up fund for Excellent Scholarship of the Chinese Academy of Sciences (07YQ091001) (to Y-GH). We would like to thank Jessie Lai for laboratory support at The University of Hong Kong.
PY - 2011/2
Y1 - 2011/2
N2 - We investigated the diversity, spatial distribution, and abundances of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in sediment samples of different depths collected from a transect with different distances to mangrove forest in the territories of Hong Kong. Both the archaeal and bacterial amoA genes (encoding ammonia monooxygenase subunit A) from all samples supported distinct phylogenetic groups, indicating the presences of niche-specific AOA and AOB in mangrove sediments. The higher AOB abundances than AOA in mangrove sediments, especially in the vicinity of the mangrove trees, might indicate the more important role of AOB on nitrification. The spatial distribution showed that AOA had higher diversity and abundance in the surface layer sediments near the mangrove trees (0 and 10 m) but lower away from the mangrove trees (1,000 m), and communities of AOA could be clustered into surface and bottom sediment layer groups. In contrast, AOB showed a reverse distributed pattern, and its communities were grouped by the distances between sites and mangrove trees, indicating mangrove trees might have different influences on AOA and AOB community structures. Furthermore, the strong correlations among archaeal and bacterial amoA gene abundances and their ratio with NH 4 + , salinity, and pH of sediments indicated that these environmental factors have strong influences on AOA and AOB distributions in mangrove sediments. In addition, AOA diversity and abundances were significantly correlated with hzo gene abundances, which encodes the key enzyme for transformation of hydrazine into N2 in anaerobic ammonium-oxidizing (anammox) bacteria, indicating AOA and anammox bacteria may interact with each other or they are influenced by the same controlling factors, such as NH 4 + . The results provide a better understanding on using mangrove wetlands as biological treatment systems for removal of nutrients.
AB - We investigated the diversity, spatial distribution, and abundances of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in sediment samples of different depths collected from a transect with different distances to mangrove forest in the territories of Hong Kong. Both the archaeal and bacterial amoA genes (encoding ammonia monooxygenase subunit A) from all samples supported distinct phylogenetic groups, indicating the presences of niche-specific AOA and AOB in mangrove sediments. The higher AOB abundances than AOA in mangrove sediments, especially in the vicinity of the mangrove trees, might indicate the more important role of AOB on nitrification. The spatial distribution showed that AOA had higher diversity and abundance in the surface layer sediments near the mangrove trees (0 and 10 m) but lower away from the mangrove trees (1,000 m), and communities of AOA could be clustered into surface and bottom sediment layer groups. In contrast, AOB showed a reverse distributed pattern, and its communities were grouped by the distances between sites and mangrove trees, indicating mangrove trees might have different influences on AOA and AOB community structures. Furthermore, the strong correlations among archaeal and bacterial amoA gene abundances and their ratio with NH 4 + , salinity, and pH of sediments indicated that these environmental factors have strong influences on AOA and AOB distributions in mangrove sediments. In addition, AOA diversity and abundances were significantly correlated with hzo gene abundances, which encodes the key enzyme for transformation of hydrazine into N2 in anaerobic ammonium-oxidizing (anammox) bacteria, indicating AOA and anammox bacteria may interact with each other or they are influenced by the same controlling factors, such as NH 4 + . The results provide a better understanding on using mangrove wetlands as biological treatment systems for removal of nutrients.
KW - Abundance
KW - Ammonia-oxidizing archaea (AOA)
KW - Ammonia-oxidizing bacteria (AOB)
KW - Anammox
KW - Community structures
KW - Diversity
KW - Mangrove sediments
KW - hzo gene
UR - http://www.scopus.com/inward/record.url?scp=79952532923&partnerID=8YFLogxK
U2 - 10.1007/s00253-010-2929-0
DO - 10.1007/s00253-010-2929-0
M3 - 文章
C2 - 20953601
AN - SCOPUS:79952532923
SN - 0175-7598
VL - 89
SP - 1243
EP - 1254
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 4
ER -