Spatial competition with Lactococcus lactis in mixed-species continuous-flow biofilms inhibits Listeria monocytogenes growth

Olivier Habimana, Laurent Guillier, Saulius Kulakauskas, Romain Briandet*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Surfaces in industrial settings provide a home for resident biofilms that are likely to interact with the attachment, growth and survival of pathogens such as Listeria monocytogenes. Experimental results have indicated that L. monocytogenes cells were inhibited by the presence of a model resident flora (Lactococcus lactis) in dual-species continuous flow-biofilms, and are spatially restricted to the lower biofilm layers. Using a new, simplified individualbased model (IBM) that simulates bacterial cell growth in a three-dimensional space, the spatial arrangements of the two species were reconstructed and their cell counts successfully predicted. This model showed that the difference in generation times between L. monocytogenes and L. lactis cells during the initial stages of dual-species biofilm formation was probably responsible for the species spatialization observed and the subsequent inhibition of growth of the pathogen.

Original languageEnglish
Pages (from-to)1065-1072
Number of pages8
JournalBiofouling
Volume27
Issue number9
DOIs
StatePublished - Oct 2011
Externally publishedYes

Keywords

  • Flow-biofilm
  • Individual-based model
  • Lactococcus lactis
  • Listeria monocytogenes
  • Spatial competition

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