Screening, isolation and optimization of an extracellular lipase producing Exiguobacterium sp. BBXS-7 segregated from waste cooking oil contaminated sites

Chaudhry Haider Ali, Jing-Jing Zhang, Serge Maurice Mbadinga, Jin-Feng Liu, Shizhong Yang, Ji-Dong Gu, Bo-Zhong Mu

Research output: Contribution to journalArticlepeer-review

Abstract

Lipases are enzymes known for their specific hydrolysis of acyl glycerol, greases and oils. In the present work, we have aimed to isolate a new lipolytic bacterium from waste cooking oils contaminated soil. The isolate was identified as Exiguobacterium sp. by means of 16S rRNA gene sequencing analysis. The production of lipase was carried out in a shake flask mode. Optimization of culture conditions showed an overall 3.62 fold improvement in lipase activity (26.654 U/mL). Olive oil and yeast extract were found to be the most suitable substrates for maximum lipase production. Lipase activity was inhibited by Fe2+ and Al3+ whereas Ca2+ ions enhance it. The enzyme lost its activity with 0.1% SDS and Triton X-100; whereas tween 20 and tween 80 have improved the activity. The findings of the present study have important biotechnological interests because microorganisms of the genus Exiguobacterium can be targeted as potential candidates for industrial applications.
Original languageEnglish
Pages (from-to)183-201
Number of pages19
JournalKärntner Botanikzentrum
Volume22
StatePublished - 3 May 2015
Externally publishedYes

Keywords

  • Exiguobacterium sp. BBXS-7
  • lipase activity
  • medium optimization
  • strain isolation
  • barbeque sites
  • Phylogenetic analysis

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