TY - JOUR
T1 - Residence of Habitat-Specific Anammox Bacteria in the Deep-Sea Subsurface Sediments of the South China Sea
T2 - Analyses of Marker Gene Abundance with Physical Chemical Parameters
AU - Hong, Yi Guo
AU - Li, Meng
AU - Cao, Huiluo
AU - Gu, Ji Dong
N1 - Funding Information:
This research was supported by the National Natural Science Foundation of China (30800032, 41076095); PhD studentship (ML) and in part by the Agriculture, Fisheries and Conservation Department of the Hong Kong SAR Government (J-DG); the Knowledge Innovation Key Project of The Chinese Academy of Sciences (KZCX2-YW-QN207); and South China Sea Open Cruise by R/V Shiyan 3, South China Sea Institute of Oceanography, CAS. We thank Jessie Lai for general laboratory assistance during this study at The University of Hong Kong.
PY - 2011/7
Y1 - 2011/7
N2 - Anaerobic ammonium oxidation (anammox) has been recognized as an important process for the global nitrogen cycle. In this study, the occurrence and diversity of anammox bacteria in the deep-sea subsurface sediments of the South China Sea (SCS) were investigated. Results indicated that the anammox bacterial sequences recovered from this habitat by amplifying both 16S rRNA gene and hydrazine oxidoreductase encoding hzo gene were all closely related to the Candidatus Scalindua genus. A total of 96 16S rRNA gene sequences from 346 clones were grouped into five subclusters: two subclusters affiliated with the brodae and arabica species, while three new subclusters named zhenghei-I, -II, and -III showed ≤97.4% nucleic acid sequence identity with other known Candidatus Scalindua species. Meanwhile, 88 hzo gene sequences from the sediments also formed five distant subclusters within hzo cluster 1c. Through fluorescent real-time PCR analysis, the abundance of anammox bacteria in deep-sea subsurface sediment was quantified by hzo genes, which ranged from 1.19 × 104 to 7.17 × 104 copies per gram of dry sediments. Combining all the information from this study, diverse Candidatus Scalindua anammox bacteria were found in the deep-sea subsurface sediments of the SCS, and they could be involved in the nitrogen loss from the fixed inventory in the habitat.
AB - Anaerobic ammonium oxidation (anammox) has been recognized as an important process for the global nitrogen cycle. In this study, the occurrence and diversity of anammox bacteria in the deep-sea subsurface sediments of the South China Sea (SCS) were investigated. Results indicated that the anammox bacterial sequences recovered from this habitat by amplifying both 16S rRNA gene and hydrazine oxidoreductase encoding hzo gene were all closely related to the Candidatus Scalindua genus. A total of 96 16S rRNA gene sequences from 346 clones were grouped into five subclusters: two subclusters affiliated with the brodae and arabica species, while three new subclusters named zhenghei-I, -II, and -III showed ≤97.4% nucleic acid sequence identity with other known Candidatus Scalindua species. Meanwhile, 88 hzo gene sequences from the sediments also formed five distant subclusters within hzo cluster 1c. Through fluorescent real-time PCR analysis, the abundance of anammox bacteria in deep-sea subsurface sediment was quantified by hzo genes, which ranged from 1.19 × 104 to 7.17 × 104 copies per gram of dry sediments. Combining all the information from this study, diverse Candidatus Scalindua anammox bacteria were found in the deep-sea subsurface sediments of the SCS, and they could be involved in the nitrogen loss from the fixed inventory in the habitat.
UR - http://www.scopus.com/inward/record.url?scp=79960583718&partnerID=8YFLogxK
U2 - 10.1007/s00248-011-9849-0
DO - 10.1007/s00248-011-9849-0
M3 - 文章
C2 - 21491114
AN - SCOPUS:79960583718
SN - 0095-3628
VL - 62
SP - 36
EP - 47
JO - Microbial Ecology
JF - Microbial Ecology
IS - 1
ER -