Rapid identification of betacyanins from Amaranthus tricolor, Gomphrena globosa, and Hylocereus polyrhizus by matrix-assisted laser desorption/ ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS)

TY - JOUR

T1 - Rapid identification of betacyanins from Amaranthus tricolor, Gomphrena globosa, and Hylocereus polyrhizus by matrix-assisted laser desorption/ ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS)

AU - Cai, Yi Zhong

AU - Xing, Jie

AU - Sun, Mei

AU - Corke, Harold

PY - 2006/9/6

Y1 - 2006/9/6

N2 - Natural betacyanins have attracted great attention as food colorants and potential antioxidants. Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) is a new and powerful technique for the identification of low molecular weight compounds. This study is the first to employ MALDI-QIT-TOF MS to rapidly identify, within a few minutes, a great number of betacyanins in crude extracts from Amaranthus tricolor seedlings, Gomphrena globosa flowers, and Hylocereus polyrhizus fruits. The fresh crude extract samples without any purification were directly used for MALDI-QIT-TOF MS analysis with 2,5-dihydroxybenzoic acid as a matrix. The MS 2 and MS 3 spectrometric data acquired could provide important characteristic information for structural elucidation of the betacyanins. Fourteen free and acylated betacyanins, belonging to amaranthin-type, betanin-type, and gomphrenin-type betacyanins, respectively, were identified. However, the related isomers should be differentiated with the aid of HPLC.

AB - Natural betacyanins have attracted great attention as food colorants and potential antioxidants. Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) is a new and powerful technique for the identification of low molecular weight compounds. This study is the first to employ MALDI-QIT-TOF MS to rapidly identify, within a few minutes, a great number of betacyanins in crude extracts from Amaranthus tricolor seedlings, Gomphrena globosa flowers, and Hylocereus polyrhizus fruits. The fresh crude extract samples without any purification were directly used for MALDI-QIT-TOF MS analysis with 2,5-dihydroxybenzoic acid as a matrix. The MS 2 and MS 3 spectrometric data acquired could provide important characteristic information for structural elucidation of the betacyanins. Fourteen free and acylated betacyanins, belonging to amaranthin-type, betanin-type, and gomphrenin-type betacyanins, respectively, were identified. However, the related isomers should be differentiated with the aid of HPLC.

KW - Amaranth

KW - Amaranthus tricolor, gomphrena globosa

KW - Betacyanins

KW - Betalains

KW - Food colorants

KW - HPLC

KW - Hylocereus polyrhizus

KW - Low molecular weight compounds

KW - MALDI-QIT-TOF MS

KW - Pitaya

UR - http://www.scopus.com/inward/record.url?scp=33748911624&partnerID=8YFLogxK

U2 - 10.1021/jf0609983

DO - 10.1021/jf0609983

M3 - 文章

C2 - 16939305

AN - SCOPUS:33748911624

SN - 0021-8561

VL - 54

SP - 6520

EP - 6526

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 18

ER -