@inbook{9f7e9c67bae54593aa9fcc2b5acd3bbb,
title = "Protein profiles of extracellular polymeric substances and activated sludge in a membrane biological reactor by 2-dimensional gel electrophoresis",
abstract = "Extracellular polymeric substances (EPS) play a key role in wastewater treatment, specifically flocculation, settling and treatment efficiency. Proteins are the predominant components in EPS and influence hydrophobicity and flocculation of sludge. In this paper, two-dimensional gel electrophoresis (2-DGE) technique was used to analyse protein profiles of EPS from a membrane biological reactor (MBR) fed either glucose or acetate. A highly reproducible 2-DGE protocol was developed achieving >95% of protein spots matches in repeated runs. Results also showed that proteins of EPS obtained from the activated sludge displayed a similar protein pattern between pH 4-7 with molecular weight 10-80 kD. Protein profiles of EPS extracted from activated sludge fed glucose and acetate showed similar distribution patterns for proteins, but further separation of proteins between pH 4-7 revealed distinctive differences between the specific proteins from these two carbon sources. In addition, information of 2-DGE protein profiles indicates little relationship between the physical and chemical analyses. It is apparent that 2-DGE is a much more powerful and a very useful tool in studying metabolisms of microbial communities at the level of metaproteomics.",
keywords = "Activated sludge, Extracellular polymeric substances (EPS), Membrane biological reactor (MBR), Two-dimensional gel electrophoresis (2-DGE)",
author = "B. Xie and Gu, {J. D.} and Li, {X. Y.}",
year = "2006",
doi = "10.2166/ws.2006.968",
language = "英语",
isbn = "1843395908",
series = "Water Science and Technology: Water Supply",
number = "6",
pages = "27--33",
booktitle = "Leading-edge Strategies and Technologies for Sustainable Urban Water Management",
edition = "6",
}