Potential use of Raman spectroscopy for determination of amylose content in maize starch

David Lee Phillips*, Jie Xing, Huijun Liu, Duo Hai Pan, Harold Corke

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

An analytical method using Raman spectroscopy was developed for the determination of amylose concentration in maize starches. FT-Raman spectra of four maize starches with amylose content varying from 3.3 to 66% were obtained. A Raman band at ≃1657 cm-1 correlated linearly with amylose concentration in the four maize starches, and a calibration curve for Raman band intensity versus amylose content was developed. The linear correlation of the I1657/I900 integrated areas with amylose content was r = 0.997. The Raman-based calibration curve allows fast and nondestructive determination of the amylose content in maize starches with minimal sample preparation.

Original languageEnglish
Pages (from-to)821-823
Number of pages3
JournalCereal Chemistry
Volume76
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

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