Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models (Final Report)

Gregory Stephanopoulos, James Liao, Jens Nielsen, Scott Baker, Andreas Vasdekis, Adrian Fay, Kangjian Qiao, Andrew Silverman, Nian Liu, Peng Xu, Thomas Wasylenko, Hongjuan Liu, Sagar Chakraborty, Jimmy Lafontaine Rivera, Yun Lee, Po-Wei Chen, Matthew Theisen, Eduard Kerkhoven, Michael Gossing, Kyle PomraningErin Bredeweg, Ziyu Dai, Sue Karagiosis

Research output: Book/ReportCommissioned report


The work funded under this DOE genomics grant focused on characterizing, modifying and developing genetic engineering tools for the then very promising but under-characterized oleaginous yeast Yarrowia lipolytica. To-date, all biodiesel production facilities rely on vegetable oils and animal fats as feedstocks, which are very limited. Nature, on the other hand, is very well equipped for making carbohydrates, which are very plentiful throughout the world and rather well distributed in various forms. While numerous (biochemical and thermochemical) technologies exist presently for carbohydrate conversion to alcohols, there is none available for the cost-efficient production of lipids from carbohydrate feedstocks. Such a technology would have wide-ranging implications in land use, renewable resource utilization and production of transportation fuels with minimal carbon footprint. The global research objectives of this award were to develop tools for cell-wide measurement of metabolites and lipids that, along with transcriptional data, will allow the construction of genome-scale metabolic models, as well as models of transcriptional regulation, that will guide the further metabolic engineering of Yarrowia lipolytica. To achieve these goals, a very strong international and diverse team consisting of research groups at MIT, UCLA, PNNL and Chalmers University was put together, whose specific goals, achievements and breakthroughs are included in this report.
Original languageEnglish
PublisherOffice of Scientific and Technical Information (OSTI)
StateSubmitted - 20 Sep 2021
Externally publishedYes


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