One-stage partial nitritation and anammox in membrane bioreactor

TY - JOUR

T1 - One-stage partial nitritation and anammox in membrane bioreactor

AU - Huang, Xiaowu

AU - Sun, Kaihang

AU - Wei, Qiaoyan

AU - Urata, Kohei

AU - Yamashita, Yuki

AU - Hong, Nian

AU - Hama, Takehide

AU - Kawagoshi, Yasunori

N1 - Publisher Copyright: © 2016, Springer-Verlag Berlin Heidelberg.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Partial nitritation and anammox (PN/A) was applied in a lab-scale membrane bioreactor (MBR) to investigate its technical feasibility for treating ammonium-rich wastewater with low C/N ratio. The bacterial community was analyzed by molecular cloning and 16S rRNA sequence analysis. Partial nitritation (PN) was first realized in MBR by seeding aerobic activated sludge. With dissolved oxygen control, a steady effluent mixture with NO2 −-N/NH4 +-N ratio of 1.13 ± 0.08 was generated from the PN process. Subsequently, the MBR was seeded with anammox biomass on day 59. After running 300 days, the one-stage PN/A achieved a maximum nitrogen removal rate of 1.45 kg N/m3/day at the nitrogen removal efficiency of 89.5 %. Microbial community analysis revealed that Nitrosomonas sp. HKU and Nitrosospira sp. YKU corresponded to nitritation; meanwhile, Candidatus Brocadia TKU sp. accounted for nitrogen removal of the PN/A system. Specifically, Nitrosomonas sp. were enriched in the reactor at the PN/A phase and then conquered Nitrosospira sp. to be the predominant ammonia oxidizers. Nitrite oxidizers and denitrifiers were detected in symbiosis with aforementioned microbes. Denitrification promised potential plus nitrogen depletion. The present one-stage PN/A process allows a significant decrease in operational costs compared with classical nitrification/denitrification.

AB - Partial nitritation and anammox (PN/A) was applied in a lab-scale membrane bioreactor (MBR) to investigate its technical feasibility for treating ammonium-rich wastewater with low C/N ratio. The bacterial community was analyzed by molecular cloning and 16S rRNA sequence analysis. Partial nitritation (PN) was first realized in MBR by seeding aerobic activated sludge. With dissolved oxygen control, a steady effluent mixture with NO2 −-N/NH4 +-N ratio of 1.13 ± 0.08 was generated from the PN process. Subsequently, the MBR was seeded with anammox biomass on day 59. After running 300 days, the one-stage PN/A achieved a maximum nitrogen removal rate of 1.45 kg N/m3/day at the nitrogen removal efficiency of 89.5 %. Microbial community analysis revealed that Nitrosomonas sp. HKU and Nitrosospira sp. YKU corresponded to nitritation; meanwhile, Candidatus Brocadia TKU sp. accounted for nitrogen removal of the PN/A system. Specifically, Nitrosomonas sp. were enriched in the reactor at the PN/A phase and then conquered Nitrosospira sp. to be the predominant ammonia oxidizers. Nitrite oxidizers and denitrifiers were detected in symbiosis with aforementioned microbes. Denitrification promised potential plus nitrogen depletion. The present one-stage PN/A process allows a significant decrease in operational costs compared with classical nitrification/denitrification.

KW - Anammox

KW - Membrane bioreactor

KW - Nitrogen removal

KW - Partial nitritation

UR - http://www.scopus.com/inward/record.url?scp=84971351803&partnerID=8YFLogxK

U2 - 10.1007/s11356-016-6309-x

DO - 10.1007/s11356-016-6309-x

M3 - 文章

C2 - 26916267

AN - SCOPUS:84971351803

SN - 0944-1344

VL - 23

SP - 11149

EP - 11162

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

IS - 11

ER -