Molecular detection, quantification and distribution of alkane-degrading bacteria in production water from low temperature oilfields

TY - JOUR

T1 - Molecular detection, quantification and distribution of alkane-degrading bacteria in production water from low temperature oilfields

AU - Li, Hui

AU - Wang, Xiao Li

AU - Mu, Bo Zhong

AU - Gu, Ji Dong

AU - Liu, Yong Di

AU - Lin, Kuang Fei

AU - Lu, Shu Guang

AU - Lu, Qiang

AU - Li, Bing Zhi

AU - Li, Yang Yang

AU - Du, Xiao Ming

N1 - Funding Information: This study was financially supported by the National Natural Science Foundation of China ( 40871223 , 41003031 , 41073055 ), the National Environmental Protection Public Welfare Science and Technology Research Program of China ( 200909089 , 2010467032 , 201109013 ), the Specialized Research Fund for the Doctoral Program of Higher Education ( 20110074130002 ), the Fundamental Research Funds for the Central Universities ( WB1014004 ), the Major Science and Technology Program for Water Pollution Control and Treatment ( 2009ZX07101-009-03 ), the National Science Foundation for Postdoctoral Scientists of China ( 2012M510811 ), and the Postdoctoral Science Foundation of Shanghai ( 11R21412500 ). We also would like to thank the anonymous referees for their helpful comments on this paper.

PY - 2013/1

Y1 - 2013/1

N2 - Alkane-degrading bacteria are crucial in the bioremediation of petroleum contamination from soil and groundwater. The alkane monooxygenase (alkB) gene encoding the Alk enzyme involved in aerobic degradation is a potentially functional gene biomarker for the detection of alkane-degrading bacteria. This study describes a denaturing gradient gel electrophoresis (DGGE) assay to facilitate the rapid and sensitive detection of alkB genes in wastewater from oilfield. The results showed that the presence of a considerable genetic diversity of alkB genes in the wastewater as evidenced by a total of 13 unique DNA bands detected. These alkB genes belong to nine genera, including Acinetobacter, Alcanivorax, Acidisphaera, Burkholderia, Geobacillus, Marinobacter, Mycobacterium, Pseudomonas, Rhodococcus and Xanthobacter. The abundance of alkane-degrading bacteria and total bacteria was calculated to range from 1.46 × 103 to 9.89 × 104 cell ml-1 and from 1.18 × 104 to 6.29 × 105 cells ml-1, respectively. The distribution of alkane-degrading bacteria was positively correlated with the environmental temperature and the specific types of n-alkanes in the wastewaters. Our results suggest that alkB-based DGGE methods could describe the diverse alkane-degrading bacteria in oil-degrading community, which may improve the understanding of the treatment for oil-polluted wastewater.

AB - Alkane-degrading bacteria are crucial in the bioremediation of petroleum contamination from soil and groundwater. The alkane monooxygenase (alkB) gene encoding the Alk enzyme involved in aerobic degradation is a potentially functional gene biomarker for the detection of alkane-degrading bacteria. This study describes a denaturing gradient gel electrophoresis (DGGE) assay to facilitate the rapid and sensitive detection of alkB genes in wastewater from oilfield. The results showed that the presence of a considerable genetic diversity of alkB genes in the wastewater as evidenced by a total of 13 unique DNA bands detected. These alkB genes belong to nine genera, including Acinetobacter, Alcanivorax, Acidisphaera, Burkholderia, Geobacillus, Marinobacter, Mycobacterium, Pseudomonas, Rhodococcus and Xanthobacter. The abundance of alkane-degrading bacteria and total bacteria was calculated to range from 1.46 × 103 to 9.89 × 104 cell ml-1 and from 1.18 × 104 to 6.29 × 105 cells ml-1, respectively. The distribution of alkane-degrading bacteria was positively correlated with the environmental temperature and the specific types of n-alkanes in the wastewaters. Our results suggest that alkB-based DGGE methods could describe the diverse alkane-degrading bacteria in oil-degrading community, which may improve the understanding of the treatment for oil-polluted wastewater.

KW - AlkB gene

KW - Alkane-degrading bacteria

KW - Bioremediation

KW - Denaturing gradient gel electrophoresis (DGGE)

KW - Oil contamination

UR - http://www.scopus.com/inward/record.url?scp=84871808228&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2012.06.007

DO - 10.1016/j.ibiod.2012.06.007

M3 - 文章

AN - SCOPUS:84871808228

SN - 0964-8305

VL - 76

SP - 49

EP - 57

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

ER -