Microbial community composition and diversity in production water of a high-temperature offshore oil reservoir assessed by DNA- and RNA-based analyses

Lei Zhou; Yu Wei Lu; Da Wei Wang; Shi Lun Zhang; En Gao Tang; Zhen Zhen Qi; Sheng Nan Xie; Jun Wu; Bo Liang; Jin Feng Liu; Shi Zhong Yang; Jian Zhang; Ji Dong Gu; Bo Zhong Mu

doi:10.1016/j.ibiod.2020.104970

Microbial community composition and diversity in production water of a high-temperature offshore oil reservoir assessed by DNA- and RNA-based analyses

Lei Zhou, Yu Wei Lu, Da Wei Wang, Shi Lun Zhang, En Gao Tang, Zhen Zhen Qi, Sheng Nan Xie, Jun Wu, Bo Liang, Jin Feng Liu, Shi Zhong Yang, Jian Zhang, Ji Dong Gu, Bo Zhong Mu^*

^*Corresponding author for this work

Environmental Science and Engineering

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Microbial activity is ubiquitous and important in petroleum reservoir systems; however, current knowledge regarding the diversity and functions of microbial communities that are active in offshore petroleum reservoirs remains very limited. In the present work, high-throughput sequencing analysis of the 16S rRNA gene (DNA) and transcripts (RNA) was applied to respectively characterize the genomic and active microbial communities from two production wells in a high-temperature offshore oilfield in China. The results showed that the microbial community compositions in the two wells (X1 and X2) were very different. At the genus level, the dominant members in both genomic and active communities of sample X1 were Bosea, Acinetobacter, Sphingomonas, Candidatus Methanofastidiosum and Methanocalculus. Both genomic and active communities in sample X2 were mainly represented by Pseudomonas, Acinetobacter, Methanothermobacter, Methanosaeta, Candidatus Nitrosopumilus, Candidatus Methanomethylicus, Thermococcus, Candidatus Methanofastidiosum and Methanocalculus. Moreover, using an RNA-based approach, genera with a very low abundance and even undetected genera (based on a DNA approach) were shown to have increased activity. Besides the abundant active members, the comparison between the DNA- and RNA-based datasets also permitted the identification of genera that were relatively more active in the two production water samples. The existence of these active microorganisms suggested they might have potential functions associated with reservoir issues in the offshore oilfield. This study highlights the importance of assessing microbial diversity through a combination of DNA- and RNA-based analyses, and promotes a comprehensive and deep understanding of microbial compositions and their roles in offshore petroleum reservoirs.

Original language	English
Article number	104970
Journal	International Biodeterioration and Biodegradation
Volume	151
DOIs	https://doi.org/10.1016/j.ibiod.2020.104970
State	Published - Jul 2020

Keywords

16S rRNA sequencing
Active community
DNA- and RNA-Based
Microbial community
Offshore petroleum reservoir

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10.1016/j.ibiod.2020.104970

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Zhou, L., Lu, Y. W., Wang, D. W., Zhang, S. L., Tang, E. G., Qi, Z. Z., Xie, S. N., Wu, J., Liang, B., Liu, J. F., Yang, S. Z., Zhang, J., Gu, J. D., & Mu, B. Z. (2020). Microbial community composition and diversity in production water of a high-temperature offshore oil reservoir assessed by DNA- and RNA-based analyses. International Biodeterioration and Biodegradation, 151, Article 104970. https://doi.org/10.1016/j.ibiod.2020.104970

@article{4bb104b1063f4730918fcaa2ec4b7a84,

title = "Microbial community composition and diversity in production water of a high-temperature offshore oil reservoir assessed by DNA- and RNA-based analyses",

abstract = "Microbial activity is ubiquitous and important in petroleum reservoir systems; however, current knowledge regarding the diversity and functions of microbial communities that are active in offshore petroleum reservoirs remains very limited. In the present work, high-throughput sequencing analysis of the 16S rRNA gene (DNA) and transcripts (RNA) was applied to respectively characterize the genomic and active microbial communities from two production wells in a high-temperature offshore oilfield in China. The results showed that the microbial community compositions in the two wells (X1 and X2) were very different. At the genus level, the dominant members in both genomic and active communities of sample X1 were Bosea, Acinetobacter, Sphingomonas, Candidatus Methanofastidiosum and Methanocalculus. Both genomic and active communities in sample X2 were mainly represented by Pseudomonas, Acinetobacter, Methanothermobacter, Methanosaeta, Candidatus Nitrosopumilus, Candidatus Methanomethylicus, Thermococcus, Candidatus Methanofastidiosum and Methanocalculus. Moreover, using an RNA-based approach, genera with a very low abundance and even undetected genera (based on a DNA approach) were shown to have increased activity. Besides the abundant active members, the comparison between the DNA- and RNA-based datasets also permitted the identification of genera that were relatively more active in the two production water samples. The existence of these active microorganisms suggested they might have potential functions associated with reservoir issues in the offshore oilfield. This study highlights the importance of assessing microbial diversity through a combination of DNA- and RNA-based analyses, and promotes a comprehensive and deep understanding of microbial compositions and their roles in offshore petroleum reservoirs.",

keywords = "16S rRNA sequencing, Active community, DNA- and RNA-Based, Microbial community, Offshore petroleum reservoir",

author = "Lei Zhou and Lu, {Yu Wei} and Wang, {Da Wei} and Zhang, {Shi Lun} and Tang, {En Gao} and Qi, {Zhen Zhen} and Xie, {Sheng Nan} and Jun Wu and Bo Liang and Liu, {Jin Feng} and Yang, {Shi Zhong} and Jian Zhang and Gu, {Ji Dong} and Mu, {Bo Zhong}",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = jul,

doi = "10.1016/j.ibiod.2020.104970",

language = "英语",

volume = "151",

journal = "International Biodeterioration and Biodegradation",

issn = "0964-8305",

publisher = "Elsevier Ltd.",

}

Zhou, L, Lu, YW, Wang, DW, Zhang, SL, Tang, EG, Qi, ZZ, Xie, SN, Wu, J, Liang, B, Liu, JF, Yang, SZ, Zhang, J, Gu, JD & Mu, BZ 2020, 'Microbial community composition and diversity in production water of a high-temperature offshore oil reservoir assessed by DNA- and RNA-based analyses', International Biodeterioration and Biodegradation, vol. 151, 104970. https://doi.org/10.1016/j.ibiod.2020.104970

TY - JOUR

T1 - Microbial community composition and diversity in production water of a high-temperature offshore oil reservoir assessed by DNA- and RNA-based analyses

AU - Zhou, Lei

AU - Lu, Yu Wei

AU - Wang, Da Wei

AU - Zhang, Shi Lun

AU - Tang, En Gao

AU - Qi, Zhen Zhen

AU - Xie, Sheng Nan

AU - Wu, Jun

AU - Liang, Bo

AU - Liu, Jin Feng

AU - Yang, Shi Zhong

AU - Zhang, Jian

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

PY - 2020/7

Y1 - 2020/7

N2 - Microbial activity is ubiquitous and important in petroleum reservoir systems; however, current knowledge regarding the diversity and functions of microbial communities that are active in offshore petroleum reservoirs remains very limited. In the present work, high-throughput sequencing analysis of the 16S rRNA gene (DNA) and transcripts (RNA) was applied to respectively characterize the genomic and active microbial communities from two production wells in a high-temperature offshore oilfield in China. The results showed that the microbial community compositions in the two wells (X1 and X2) were very different. At the genus level, the dominant members in both genomic and active communities of sample X1 were Bosea, Acinetobacter, Sphingomonas, Candidatus Methanofastidiosum and Methanocalculus. Both genomic and active communities in sample X2 were mainly represented by Pseudomonas, Acinetobacter, Methanothermobacter, Methanosaeta, Candidatus Nitrosopumilus, Candidatus Methanomethylicus, Thermococcus, Candidatus Methanofastidiosum and Methanocalculus. Moreover, using an RNA-based approach, genera with a very low abundance and even undetected genera (based on a DNA approach) were shown to have increased activity. Besides the abundant active members, the comparison between the DNA- and RNA-based datasets also permitted the identification of genera that were relatively more active in the two production water samples. The existence of these active microorganisms suggested they might have potential functions associated with reservoir issues in the offshore oilfield. This study highlights the importance of assessing microbial diversity through a combination of DNA- and RNA-based analyses, and promotes a comprehensive and deep understanding of microbial compositions and their roles in offshore petroleum reservoirs.

AB - Microbial activity is ubiquitous and important in petroleum reservoir systems; however, current knowledge regarding the diversity and functions of microbial communities that are active in offshore petroleum reservoirs remains very limited. In the present work, high-throughput sequencing analysis of the 16S rRNA gene (DNA) and transcripts (RNA) was applied to respectively characterize the genomic and active microbial communities from two production wells in a high-temperature offshore oilfield in China. The results showed that the microbial community compositions in the two wells (X1 and X2) were very different. At the genus level, the dominant members in both genomic and active communities of sample X1 were Bosea, Acinetobacter, Sphingomonas, Candidatus Methanofastidiosum and Methanocalculus. Both genomic and active communities in sample X2 were mainly represented by Pseudomonas, Acinetobacter, Methanothermobacter, Methanosaeta, Candidatus Nitrosopumilus, Candidatus Methanomethylicus, Thermococcus, Candidatus Methanofastidiosum and Methanocalculus. Moreover, using an RNA-based approach, genera with a very low abundance and even undetected genera (based on a DNA approach) were shown to have increased activity. Besides the abundant active members, the comparison between the DNA- and RNA-based datasets also permitted the identification of genera that were relatively more active in the two production water samples. The existence of these active microorganisms suggested they might have potential functions associated with reservoir issues in the offshore oilfield. This study highlights the importance of assessing microbial diversity through a combination of DNA- and RNA-based analyses, and promotes a comprehensive and deep understanding of microbial compositions and their roles in offshore petroleum reservoirs.

KW - 16S rRNA sequencing

KW - Active community

KW - DNA- and RNA-Based

KW - Microbial community

KW - Offshore petroleum reservoir

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U2 - 10.1016/j.ibiod.2020.104970

DO - 10.1016/j.ibiod.2020.104970

M3 - 文章

AN - SCOPUS:85083714092

SN - 0964-8305

VL - 151

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

M1 - 104970

ER -

Microbial community composition and diversity in production water of a high-temperature offshore oil reservoir assessed by DNA- and RNA-based analyses

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Keywords

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