TY - JOUR
T1 - Methyl lucidenate F isolated from the ethanol-soluble-acidic components of Ganoderma lucidum is a novel tyrosinase inhibitor
AU - Zhang, Liang
AU - Ding, Zhongyang
AU - Xu, Peng
AU - Wang, Yuhong
AU - Gu, Zhenghua
AU - Qian, Zhu
AU - Shi, Guiyang
AU - Zhang, Kechang
PY - 2011/6
Y1 - 2011/6
N2 - Tyrosinase is a key enzyme in the biosynthesis of melanin, and the use of inhibitors against tyrosinase can prevent hyperpigmentation by inhibiting enzymatic oxidation. However, the current use of tyrosine inhibitors is limited by their low activities and high toxicities. The aim of the present research was to develop novel whitening agents, or tyrosinase-targeted medicine, from a submerged culture of the fungus Ganoderma lucidum. Methyl lucidenate F was isolated from the ethanol-soluble-acidic components (ESACs) of G. lucidum, with the structure of ESACs elucidated via UV, LC-MS, and 13C-NMR spectral analysis. The tyrosinase inhibitory activity was measured using catechol as a substrate. Methyl lucidenate F displayed uncompetitive inhibition of the potato tyrosinase activity, for which Lineweaver-Burk plots revealed a maximum reaction rate (V max) of 0.4367/min, Michaelis constant (K m) of 6.765 mM and uncompetitive inhibition constant (K i) of 19.22 μM. Meanwhile, methyl lucidenate F (tetra cyclic triterpenoid) exhibited high tyrosinase inhibitory activity, with an IC 50 of 32.23 μM. These results suggest that methyl lucidenate F may serve as a potential candidate for skin-whitening agents.
AB - Tyrosinase is a key enzyme in the biosynthesis of melanin, and the use of inhibitors against tyrosinase can prevent hyperpigmentation by inhibiting enzymatic oxidation. However, the current use of tyrosine inhibitors is limited by their low activities and high toxicities. The aim of the present research was to develop novel whitening agents, or tyrosinase-targeted medicine, from a submerged culture of the fungus Ganoderma lucidum. Methyl lucidenate F was isolated from the ethanol-soluble-acidic components (ESACs) of G. lucidum, with the structure of ESACs elucidated via UV, LC-MS, and 13C-NMR spectral analysis. The tyrosinase inhibitory activity was measured using catechol as a substrate. Methyl lucidenate F displayed uncompetitive inhibition of the potato tyrosinase activity, for which Lineweaver-Burk plots revealed a maximum reaction rate (V max) of 0.4367/min, Michaelis constant (K m) of 6.765 mM and uncompetitive inhibition constant (K i) of 19.22 μM. Meanwhile, methyl lucidenate F (tetra cyclic triterpenoid) exhibited high tyrosinase inhibitory activity, with an IC 50 of 32.23 μM. These results suggest that methyl lucidenate F may serve as a potential candidate for skin-whitening agents.
KW - Ganoderma lucidum
KW - Inhibitor
KW - Methyl lucidenate F
KW - Submerged culture
KW - Tyrosinase
UR - http://www.scopus.com/inward/record.url?scp=80052469857&partnerID=8YFLogxK
U2 - 10.1007/s12257-010-0345-z
DO - 10.1007/s12257-010-0345-z
M3 - 文章
AN - SCOPUS:80052469857
SN - 1226-8372
VL - 16
SP - 457
EP - 461
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
IS - 3
ER -