Metabolism of diethyl phthalate (DEP) and identification of degradation intermediates by Pseudomonas sp. DNE-S1

Yue Tao, Hanxu Li, Jidong Gu, Hongtao Shi, Siyue Han, Yaqi Jiao, Guanyu Zhong, Qi Zhang, Modupe S. Akindolie, Yulong Lin, Zhaobo Chen, Ying Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A Pseudomonas sp. DNE-S1 (GenBank accession number MF803832), able to degrade DEP in a wide range of acid-base conditions, was isolated from landfill soil. The growth kinetics of DNE-S1 on DEP followed the inhibition model. Fe 3+ could promote the degradation ability of DNE-S1 to DEP probably by over-expression of the gene phthalate dihydrodiol dehydrogenase (ophB) and phthalate dioxygenase ferredoxin reductase (ophA4). The degradation rate of DEP (500 mg L −1 at 12 h) increased by 14.5% in the presence of Fe 3+ . Cu 2+ , Zn 2+ , and Mn 2+ showed an inhibiting effect on the degradation performance of the strain and could alter the cellular morphology, surface area and volume of DNE-S1. Three degradation intermediates, namely ethyl methyl phthalate (EMP), dimethyl phthalate (DMP), and phthalic acid (PA), were detected in the biodegradation of DEP, and the biochemical pathway of DEP degradation was proposed. This study provides new information on the biochemical pathways and the responsible genes involved in DEP degradation.

Original languageEnglish
Pages (from-to)411-418
Number of pages8
JournalEcotoxicology and Environmental Safety
Volume173
DOIs
StatePublished - 30 May 2019
Externally publishedYes

Keywords

  • Biodegradation
  • Cell morphology
  • DEP
  • Inhibition kinetics
  • Microelements

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