Metabolism and biochemical pathway of n-butyl benzyl phthalate by Pseudomonas fluorescens B-1 isolated from a mangrove sediment

Xiang Rong Xu, Hua Bin Li, Ji Dong Gu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

n-Butyl benzyl phthalate (BBP) is an endocrine-disrupting chemical. Biodegradation of BBP was investigated using the bacterium Pseudomonas fluorescens B-1 isolated from mangrove sediment of Mai Po Nature Reserve of Hong Kong. The microorganism was capable of utilizing BBP as the sole source of carbon and energy while BBP was degraded in 6 days under aerobic batch culture conditions. The optimum pH, temperature, and salinity for BBP degradation by P. fluorescens B-1 was found to be 7.0, 37 °C, and 15‰, respectively. Biodegradation of BBP was fitted to the first-order kinetics model. The process of BBP biodegradation was monitored by reversed-phase high-performance liquid chromatography with ultra-violet detection after solid-phase extraction. The major metabolites of BBP degradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid, and benzoic acid by gas chromatography-mass spectrometry. BBP-degrading activity of P. fluorescens B-1 was found mostly in the soluble fraction associated with the smaller fragments of cellular membranes. Results suggest that mineralization of BBP can be achieved by microorganism of the mangrove environment.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalEcotoxicology and Environmental Safety
Volume68
Issue number3
DOIs
StatePublished - Nov 2007
Externally publishedYes

Keywords

  • Biodegradation
  • Endocrine-disruptor
  • Kinetics
  • n-Butyl benzyl phthalate
  • Pseudomonas fluorescens

Fingerprint Dive into the research topics of 'Metabolism and biochemical pathway of n-butyl benzyl phthalate by Pseudomonas fluorescens B-1 isolated from a mangrove sediment'. Together they form a unique fingerprint.

Cite this