Long-term cultivation and meta-omics reveal methylotrophic methanogenesis in hydrocarbon-impacted habitats

Yi-Fan Liu, Jing Chen, Zhong-Lin Liu, Zhao-Wei Hou, Bo Liang, Li-Ying Wang, Lei Zhou, Li-Bin Shou, Dan-Dan Lin, Shi-Zhong Yang, Jin-Feng Liu, Xiao-Lin Wu*, Ji-Dong Gu, Bo-Zhong Mu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The microbial conversion of alkanes to methane in hydrocarbon-contaminated environments is an intrinsic bioremediation strategy under anoxic conditions. However, the mechanism of microbial methanogenic alkane degradation is currently unclear. Under ten-years of continuous efforts, we obtained a methanogenic n-alkane-degrading (C15–C20) enrichment culture that exhibited sustained improvements in the kinetic properties of methane production. The integrated metagenomic and metatranscriptomic analyses revealed that n-alkanes were mainly attacked by members of Desulfosarcinaceae, Firmicutes, and Synergistetes using the fumarate addition strategy, and were then further degraded in a common effort by Tepidiphilus members. Meanwhile, the abundant members of Anaerolineaceae were mainly responsible for cell debris recycling. However, according to the metatranscriptomic analyses, methane was predicted to be produced mainly via H2-dependent methylotrophic methanogenesis, primarily from necromass-derived trimethylamine mediated by Methanomethyliaceae within the candidate phylum Verstraetearchaeota. These findings reveal that H2-dependent methylotrophic methanogens, as well as methylotrophic methanogens, may play important ecological roles in the carbon cycle of hydrocarbon enriched subsurface ecosystems.
Original languageEnglish
JournalEngineering
DOIs
StateE-pub ahead of print - 10 Jan 2022

Keywords

  • Methanogenic hydrocarbon degradation
  • Oily sludge
  • Bioremediation
  • Alkanes

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