Key players in the methanogenic biodegradation of n-hexadecane identified by DNA-Stable isotope probing

TY - JOUR

T1 - Key players in the methanogenic biodegradation of n-hexadecane identified by DNA-Stable isotope probing

AU - Liu, Jin Feng

AU - Zhang, Kai

AU - Liang, Bo

AU - Zhou, Zhi Chao

AU - Yang, Shi Zhong

AU - Li, Wei

AU - Hou, Zhao Wei

AU - Wu, Xiao Lin

AU - Gu, Ji Dong

AU - Mu, Bo Zhong

N1 - Publisher Copyright: © 2019 Elsevier Ltd

PY - 2019/9

Y1 - 2019/9

N2 - Methanogenic biodegradation of n-alkanes is an important process in the biogeochemical carbon cycles. The key players in this biochemical processes were investigated for a 920-day of culturing with universally labelled (UL)-13C-hexadecane and 1, 2-13C-hexadecane as the sources of carbon and energy, using stable isotope probing combined with 16S rRNA gene amplification. Results show that the relative abundance of Anaerolineaceae and Tepidiphilus phylotypes increased significantly in the heavy fractions of DNA obtained from the cultures amended with UL-13C-hexadecane and 1, 2-13C-hexadecane, indicating their actively involvement in the transformation and biodegradation of the alkanes by the microbial community. This study provides insights into the active members of the microbial community members in hydrocarbon degradation and is of significant scientific value in further research and relative technology to methanogenic degradation of n-alkanes.

AB - Methanogenic biodegradation of n-alkanes is an important process in the biogeochemical carbon cycles. The key players in this biochemical processes were investigated for a 920-day of culturing with universally labelled (UL)-13C-hexadecane and 1, 2-13C-hexadecane as the sources of carbon and energy, using stable isotope probing combined with 16S rRNA gene amplification. Results show that the relative abundance of Anaerolineaceae and Tepidiphilus phylotypes increased significantly in the heavy fractions of DNA obtained from the cultures amended with UL-13C-hexadecane and 1, 2-13C-hexadecane, indicating their actively involvement in the transformation and biodegradation of the alkanes by the microbial community. This study provides insights into the active members of the microbial community members in hydrocarbon degradation and is of significant scientific value in further research and relative technology to methanogenic degradation of n-alkanes.

KW - Anaerolineaceae

KW - C-labeled hexadecane

KW - DNA stable isotope probing (DNA-SIP)

KW - Methanogenic alkane degradation

KW - Tepidiphilus

UR - http://www.scopus.com/inward/record.url?scp=85066836859&partnerID=8YFLogxK

U2 - 10.1016/j.ibiod.2019.05.026

DO - 10.1016/j.ibiod.2019.05.026

M3 - 文章

AN - SCOPUS:85066836859

SN - 0964-8305

VL - 143

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

M1 - 104709

ER -