Isolation and Characterization of Three Antihypertension Peptides from the Mycelia of Ganoderma Lucidum (Agaricomycetes)

Qiang Wu, Yong Li, Kuan Peng, Xiao Ling Wang*, Zhongyang Ding, Liming Liu, Peng Xu, Gao Qiang Liu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


Ganoderma lucidum (G. lucidum) has been widely used in Asia to treat hypertension, but the active substances responsible for its antihypertensive effects remain unclear. Using the well-established angiotensin I-converting enzyme (ACE) as a target, we identified three ACE inhibitory peptides (ACEIPs), Gln-Leu-Val-Pro (QLVP), Gln-Asp-Val-Leu (QDVL), and Gln-Leu-Asp-Leu (QLDL), which account for the antihypertensive activity of G. lucidum. Notably, QLVP worked in a mixed-type manner against ACE with an IC50 value of 127.9 μmol/L. Molecular dynamics simulation suggested that the potent charge energy of QLVP, which interacted with Gln242 and Lys472 of ACE via a hydrogen bond and a salt bridge, potentially contributed to ACE inhibitory activity. Moreover, QLVP markedly activated angiotensin I-mediated phosphorylation of endothelial nitric oxide synthase in human umbilical vein endothelial cells and partly reduced mRNA and protein expression of the vasoconstrictor factor endothelin-1. This is the first report of the antihypertensive activity of small ACEIPs originating from G. lucidum mycelia, paving the way for the possible application of these peptides as potent drug candidates for treating hypertension.

Original languageEnglish
Pages (from-to)8149-8159
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Issue number29
StatePublished - 27 Jun 2019
Externally publishedYes


  • ACE inhibitory peptide
  • Ganoderma lucidum
  • antihypertension
  • molecular dynamics simulation
  • submerged fermentation


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