Isolation and characterization of diesel degrading bacteria, Sphingomonas sp. and Acinetobacter junii from petroleum contaminated soil

TY - JOUR

T1 - Isolation and characterization of diesel degrading bacteria, Sphingomonas sp. and Acinetobacter junii from petroleum contaminated soil

AU - Zhang, Qiuzhuo

AU - Wang, Duanchao

AU - Li, Mengmeng

AU - Xiang, Wei Ning

AU - Achal, Varenyam

N1 - Funding Information: Acknowledgements This work was supported by Research Innovation Fund from East China Normal University (No. 78210267), the National Natural Science Foundation of China (Grant No. 41250110527), and Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration (Grant No. 44503545).

PY - 2014/3

Y1 - 2014/3

N2 - Two indigenous bacteria of petroleum contaminated soil were characterized to utilize diesel fuel as the sole carbon and energy sources in this work. 16S rRNA gene sequence analysis identified these bacteria as Sphingomonas sp. and Acinetobacter junii. The ability to degrade diesel fuel has been demonstrated for the first time by these isolates. The results of IR analyses showed that Sphingomonas sp. VA1 and A. junii VA2 degraded up to 82.6% and 75.8% of applied diesel over 15 days, respectively. In addition, Sphingomonas sp. VA1 possessed the higher cellular hydrophobicities of 94% for diesel compared to 81% by A. junii VA2. The isolates Sphingomonas sp. VA1 and A. junii VA2 exhibited 24% and 18%, respectively emulsification activity. This study reports two new diesel degrading bacterial species, which can be effectively used for bioremediation of petroleum contaminated sites.

AB - Two indigenous bacteria of petroleum contaminated soil were characterized to utilize diesel fuel as the sole carbon and energy sources in this work. 16S rRNA gene sequence analysis identified these bacteria as Sphingomonas sp. and Acinetobacter junii. The ability to degrade diesel fuel has been demonstrated for the first time by these isolates. The results of IR analyses showed that Sphingomonas sp. VA1 and A. junii VA2 degraded up to 82.6% and 75.8% of applied diesel over 15 days, respectively. In addition, Sphingomonas sp. VA1 possessed the higher cellular hydrophobicities of 94% for diesel compared to 81% by A. junii VA2. The isolates Sphingomonas sp. VA1 and A. junii VA2 exhibited 24% and 18%, respectively emulsification activity. This study reports two new diesel degrading bacterial species, which can be effectively used for bioremediation of petroleum contaminated sites.

KW - 16S rRNA

KW - bioremediation

KW - cell surface hydrophobicity

KW - diesel degrading bacteria

KW - emulsification

UR - http://www.scopus.com/inward/record.url?scp=84894275009&partnerID=8YFLogxK

U2 - 10.1007/s11707-013-0415-6

DO - 10.1007/s11707-013-0415-6

M3 - 文章

AN - SCOPUS:84894275009

SN - 2095-0195

VL - 8

SP - 58

EP - 63

JO - Frontiers of Earth Science

JF - Frontiers of Earth Science

IS - 1

ER -