Intestinal paracellular permeability during malnutrition in guinea pigs: Effect of high dietary zinc

P. Rodríguez; N. Darmon; P. Chappuis; C. Candalh; M. A. Blaton; C. Bouchaud; M. Heyman

doi:10.1136/gut.39.3.416

Intestinal paracellular permeability during malnutrition in guinea pigs: Effect of high dietary zinc

P. Rodríguez, N. Darmon, P. Chappuis, C. Candalh, M. A. Blaton, C. Bouchaud, M. Heyman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

Background - Zinc has been shown to have beneficial effects in vitro on epithelial barrier function, and in vivo to reduce intestinal permeability in malnourished children with diarrhoea. Aims - To determine whether malnutrition alters intestinal paracellular permeability, and whether zinc prevents such alterations. Methods - Guinea pigs were fed a normal protein diet (NP group), a low protein diet (LP group), or a low protein diet enriched with 1800 ppm zinc (LPZn group) for three weeks. Intestinal permeability was measured on jejunal segments mounted in Ussing chambers by measuring ionic conductance and mucosal to serosal fluxes of ¹⁴C-mannitol, ²²Na, and horseradish peroxidase. Tight junction morphology was assessed on cryofracture replicas. Results - Mannitol and Na fluxes and ionic conductance increased in the LP group compared with the NP group but remained normal in the LPZn group. Accordingly, jejunal epithelia from the LP group, but not from the LPZn group, showed a small decrease in number of tight junctional strands compared with epithelia from the NP group. Neither malnutrition nor zinc treatment modified horseradish peroxidase fluxes. Conclusions - Malnutrition is associated with increased intestinal paracellular permeability to small molecules, and pharmacological doses of zinc prevent such functional abnormality.

Original language	English
Pages (from-to)	416-422
Number of pages	7
Journal	Gut
Volume	39
Issue number	3
DOIs	https://doi.org/10.1136/gut.39.3.416
State	Published - 1996
Externally published	Yes

Keywords

Guinea pig
Intestine
Malnutrition
Paracellular permeability
Tight junction
Zinc

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1136/gut.39.3.416

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@article{742d4aa90f834dcf92a41c2ff1694422,

title = "Intestinal paracellular permeability during malnutrition in guinea pigs: Effect of high dietary zinc",

abstract = "Background - Zinc has been shown to have beneficial effects in vitro on epithelial barrier function, and in vivo to reduce intestinal permeability in malnourished children with diarrhoea. Aims - To determine whether malnutrition alters intestinal paracellular permeability, and whether zinc prevents such alterations. Methods - Guinea pigs were fed a normal protein diet (NP group), a low protein diet (LP group), or a low protein diet enriched with 1800 ppm zinc (LPZn group) for three weeks. Intestinal permeability was measured on jejunal segments mounted in Ussing chambers by measuring ionic conductance and mucosal to serosal fluxes of 14C-mannitol, 22Na, and horseradish peroxidase. Tight junction morphology was assessed on cryofracture replicas. Results - Mannitol and Na fluxes and ionic conductance increased in the LP group compared with the NP group but remained normal in the LPZn group. Accordingly, jejunal epithelia from the LP group, but not from the LPZn group, showed a small decrease in number of tight junctional strands compared with epithelia from the NP group. Neither malnutrition nor zinc treatment modified horseradish peroxidase fluxes. Conclusions - Malnutrition is associated with increased intestinal paracellular permeability to small molecules, and pharmacological doses of zinc prevent such functional abnormality.",

keywords = "Guinea pig, Intestine, Malnutrition, Paracellular permeability, Tight junction, Zinc",

author = "P. Rodr{\'i}guez and N. Darmon and P. Chappuis and C. Candalh and Blaton, {M. A.} and C. Bouchaud and M. Heyman",

year = "1996",

doi = "10.1136/gut.39.3.416",

language = "英语",

volume = "39",

pages = "416--422",

journal = "Gut",

issn = "0017-5749",

publisher = "BMJ Publishing Group",

number = "3",

}

TY - JOUR

T1 - Intestinal paracellular permeability during malnutrition in guinea pigs

T2 - Effect of high dietary zinc

AU - Rodríguez, P.

AU - Darmon, N.

AU - Chappuis, P.

AU - Candalh, C.

AU - Blaton, M. A.

AU - Bouchaud, C.

AU - Heyman, M.

PY - 1996

Y1 - 1996

N2 - Background - Zinc has been shown to have beneficial effects in vitro on epithelial barrier function, and in vivo to reduce intestinal permeability in malnourished children with diarrhoea. Aims - To determine whether malnutrition alters intestinal paracellular permeability, and whether zinc prevents such alterations. Methods - Guinea pigs were fed a normal protein diet (NP group), a low protein diet (LP group), or a low protein diet enriched with 1800 ppm zinc (LPZn group) for three weeks. Intestinal permeability was measured on jejunal segments mounted in Ussing chambers by measuring ionic conductance and mucosal to serosal fluxes of 14C-mannitol, 22Na, and horseradish peroxidase. Tight junction morphology was assessed on cryofracture replicas. Results - Mannitol and Na fluxes and ionic conductance increased in the LP group compared with the NP group but remained normal in the LPZn group. Accordingly, jejunal epithelia from the LP group, but not from the LPZn group, showed a small decrease in number of tight junctional strands compared with epithelia from the NP group. Neither malnutrition nor zinc treatment modified horseradish peroxidase fluxes. Conclusions - Malnutrition is associated with increased intestinal paracellular permeability to small molecules, and pharmacological doses of zinc prevent such functional abnormality.

AB - Background - Zinc has been shown to have beneficial effects in vitro on epithelial barrier function, and in vivo to reduce intestinal permeability in malnourished children with diarrhoea. Aims - To determine whether malnutrition alters intestinal paracellular permeability, and whether zinc prevents such alterations. Methods - Guinea pigs were fed a normal protein diet (NP group), a low protein diet (LP group), or a low protein diet enriched with 1800 ppm zinc (LPZn group) for three weeks. Intestinal permeability was measured on jejunal segments mounted in Ussing chambers by measuring ionic conductance and mucosal to serosal fluxes of 14C-mannitol, 22Na, and horseradish peroxidase. Tight junction morphology was assessed on cryofracture replicas. Results - Mannitol and Na fluxes and ionic conductance increased in the LP group compared with the NP group but remained normal in the LPZn group. Accordingly, jejunal epithelia from the LP group, but not from the LPZn group, showed a small decrease in number of tight junctional strands compared with epithelia from the NP group. Neither malnutrition nor zinc treatment modified horseradish peroxidase fluxes. Conclusions - Malnutrition is associated with increased intestinal paracellular permeability to small molecules, and pharmacological doses of zinc prevent such functional abnormality.

KW - Guinea pig

KW - Intestine

KW - Malnutrition

KW - Paracellular permeability

KW - Tight junction

KW - Zinc

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DO - 10.1136/gut.39.3.416

M3 - 文章

C2 - 8949647

AN - SCOPUS:0029797060

SN - 0017-5749

VL - 39

SP - 416

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JO - Gut

JF - Gut

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ER -

Intestinal paracellular permeability during malnutrition in guinea pigs: Effect of high dietary zinc

Abstract

Keywords

UN SDGs

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