Increased lungkine and chitinase levels in allergic airway inflammation: A proteomics approach

Jing Zhao, Hua Zhu, Chui Hong Wong, Ka Yin Leung, W. S.Fred Wong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Asthma is a chronic inflammatory disease characterized by pulmonary eosinophilia and airway hyperresponsiveness. Mechanisms underlying the pathogenesis of asthma are still not fully understood. The present study investigated alterations in global protein expression in broncho-alveolar lavage fluid in allergic airway inflammation using a proteomics approach. BALB/c mice sensitized and challenged with ovalbumin developed airway eosinophilia, mucus hypersecretion, elevation of immunoglobulin E, and airway hyperresponsiveness. Lavage fluid proteins from normal and asthmatic mice were resolved by two-dimensional gel electrophoresis, and identified by peptide mass fingerprinting matrix-assisted laser desorption/ionization-time of flight mass spectrometry. A total of 28 protein spots were significantly altered. Several of these proteins were undetectable or at very low levels in normal mice but were significantly increased in airway inflammation. These include lungkine, a recently described chemokine, a family of chitinases including Ym1, Ym2, and acidic mammalian chitinase, gob-5, a protein that mediates mucus secretion, and surfactant protein-D, a C-type lectin capable of modulating inflammatory responses. Overall, proteomics is a powerful tool in unraveling protein expression changes in allergic airway inflammation. The proteins identified in this study may be associated with the pathogenesis of allergic airway inflammation and may also be found useful as surrogate biomarkers for asthma.

Original languageEnglish
Pages (from-to)2799-2807
Number of pages9
JournalProteomics
Volume5
Issue number11
DOIs
StatePublished - Jul 2005
Externally publishedYes

Keywords

  • Asthma
  • Bronchoalveolar lavage fluid
  • Gob-5
  • Ovalbumin
  • Surfactant protein-D

Fingerprint Dive into the research topics of 'Increased lungkine and chitinase levels in allergic airway inflammation: A proteomics approach'. Together they form a unique fingerprint.

Cite this