TY - JOUR
T1 - Hydrophobic microspheres for in situ removal of 2-phenylethanol from yeast fermentation
AU - Achmon, Yigal
AU - Goldshtein, Jenny
AU - Margel, Shlomo
AU - Fishman, Ayelet
N1 - Funding Information:
This study was supported by the Russell Berrie Nanotechnology Institute at the Technion. Partial support was also provided by a Minerva Grant (Microscale and Nanoscale Particles and Films).
PY - 2011/11
Y1 - 2011/11
N2 - The commercial production of the fragrance compound 2-phenylethanol (2-PE) from phenylalanine by yeast is limited by the accumulation of the toxic product, and therefore, in situ product removal techniques are required. We describe the use of hydrophobic polymethylmethacrylate (PMMA) microspheres of narrow size distribution of 1.53±0.10m diameter for continuous removal of 2-PE from the fermentation medium by a mechanism of swelling. In shake flask experiments with conditions simulating 2-PE stress, a 10-fold increase in productivity was measured for systems containing >10% (w/v) microspheres. A 1L fed-batch fermentation with 8% (w/v) of PMMA microspheres resulted in a total 2-PE concentration of 7.05g/L, from which 5.40g/L was incorporated inside the resin, implying 76% encapsulation. This ratio of 0.07g/g of product per resin is among the highest reported to date. Scanning electron microscopy revealed a concomitant increase in sphere diameter confirming that swelling occurred.
AB - The commercial production of the fragrance compound 2-phenylethanol (2-PE) from phenylalanine by yeast is limited by the accumulation of the toxic product, and therefore, in situ product removal techniques are required. We describe the use of hydrophobic polymethylmethacrylate (PMMA) microspheres of narrow size distribution of 1.53±0.10m diameter for continuous removal of 2-PE from the fermentation medium by a mechanism of swelling. In shake flask experiments with conditions simulating 2-PE stress, a 10-fold increase in productivity was measured for systems containing >10% (w/v) microspheres. A 1L fed-batch fermentation with 8% (w/v) of PMMA microspheres resulted in a total 2-PE concentration of 7.05g/L, from which 5.40g/L was incorporated inside the resin, implying 76% encapsulation. This ratio of 0.07g/g of product per resin is among the highest reported to date. Scanning electron microscopy revealed a concomitant increase in sphere diameter confirming that swelling occurred.
KW - 2-phenylethanol
KW - In situ product removal
KW - Polymethylmethacrylate microspheres
KW - Saccharomyces cerevisiae
KW - Single-step swelling
UR - http://www.scopus.com/inward/record.url?scp=80053393449&partnerID=8YFLogxK
U2 - 10.3109/02652048.2011.599443
DO - 10.3109/02652048.2011.599443
M3 - 文章
C2 - 21793648
AN - SCOPUS:80053393449
SN - 0265-2048
VL - 28
SP - 628
EP - 638
JO - Journal of Microencapsulation
JF - Journal of Microencapsulation
IS - 7
ER -