Highly polymorphic AFLP markers as a complementary tool to ITS sequences in assessing genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives

Junchao Huang, Harold Corke, Mei Sun*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Comparative analyses of genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives in Ipomoea series Batatas were conducted using amplified fragment length polymorphism (AFLP) and sequence data from the internal transcribed spacer (ITS) region of the ribosomal DNA. Low ITS divergence among thirteen species of ser. Batatas resulted in poorly resolved relationships. More variable AFLP characters were found to be more efficient in characterizing genetic diversity and phylogenetic relationships at both intra- and interspecific levels within ser. Batatas. Highly informative AFLP fingerprints of 36 accessions representing 10 species of ser. Batatas were generated using only six primer combinations. Of the species examined, I. trifida was found to be the most closely related to I. batatas, while I. ramosissima and I. umbraticola were the most distantly related to I. batatas. The highly polymorphic AFLP markers are a valuable tool in assessing genetic diversity and phylogenetic relationships of sweetpotato and its wild relatives.

Original languageEnglish
Pages (from-to)541-550
Number of pages10
JournalGenetic Resources and Crop Evolution
Volume49
Issue number6
DOIs
StatePublished - Dec 2002
Externally publishedYes

Keywords

  • AFLP
  • Ipomoea batatas
  • ITS
  • Phylogeny
  • Sweetpotato

Fingerprint Dive into the research topics of 'Highly polymorphic AFLP markers as a complementary tool to ITS sequences in assessing genetic diversity and phylogenetic relationships of sweetpotato (Ipomoea batatas (L.) Lam.) and its wild relatives'. Together they form a unique fingerprint.

Cite this