Green fluorescent protein-tagged Edwardsiella tarda reveals portal of entry in fish

Sharon H.M. Ling; Xian Hui Wang; Tit Meng Lim; Ka Yin Leung

doi:10.1016/S0378-1097(00)00498-5

Green fluorescent protein-tagged Edwardsiella tarda reveals portal of entry in fish

Sharon H.M. Ling, Xian Hui Wang, Tit Meng Lim, Ka Yin Leung^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

The application of green fluorescent protein (GFP) to identify the portal(s) of entry of bacterial pathogens in animal hosts was studied using the fish pathogen Edwardsiella tarda and blue gourami, Trichogaster trichopterus. An immersion challenge model was utilized to mimic natural infection conditions in fish. Gastrointestinal tract, gills and the body surface of fish were found to be the sites of entry of virulent E. tarda (PPD130/91) by histological and infection kinetics studies. On the other hand, avirulent E. tarda (PPD125/87) was mainly found in the gastrointestinal tract, and the bacterial population in tissue declined over a period of 7 days.

Original language	English
Pages (from-to)	239-243
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	194
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(00)00498-5
State	Published - 15 Jan 2001
Externally published	Yes

Keywords

Edwardsiella tarda
Green fluorescent protein
Infection kinetics
Portal of entry

Access to Document

10.1016/S0378-1097(00)00498-5

Cite this

@article{e3ed3c5c440b4c64ba468b489a42aede,

title = "Green fluorescent protein-tagged Edwardsiella tarda reveals portal of entry in fish",

abstract = "The application of green fluorescent protein (GFP) to identify the portal(s) of entry of bacterial pathogens in animal hosts was studied using the fish pathogen Edwardsiella tarda and blue gourami, Trichogaster trichopterus. An immersion challenge model was utilized to mimic natural infection conditions in fish. Gastrointestinal tract, gills and the body surface of fish were found to be the sites of entry of virulent E. tarda (PPD130/91) by histological and infection kinetics studies. On the other hand, avirulent E. tarda (PPD125/87) was mainly found in the gastrointestinal tract, and the bacterial population in tissue declined over a period of 7 days.",

keywords = "Edwardsiella tarda, Green fluorescent protein, Infection kinetics, Portal of entry",

author = "Ling, {Sharon H.M.} and Wang, {Xian Hui} and Lim, {Tit Meng} and Leung, {Ka Yin}",

note = "Funding Information: This work was supported by the Academic Research Fund of the National University of Singapore.",

year = "2001",

month = jan,

day = "15",

doi = "10.1016/S0378-1097(00)00498-5",

language = "英语",

volume = "194",

pages = "239--243",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "2",

}

TY - JOUR

T1 - Green fluorescent protein-tagged Edwardsiella tarda reveals portal of entry in fish

AU - Ling, Sharon H.M.

AU - Wang, Xian Hui

AU - Lim, Tit Meng

AU - Leung, Ka Yin

N1 - Funding Information: This work was supported by the Academic Research Fund of the National University of Singapore.

PY - 2001/1/15

Y1 - 2001/1/15

N2 - The application of green fluorescent protein (GFP) to identify the portal(s) of entry of bacterial pathogens in animal hosts was studied using the fish pathogen Edwardsiella tarda and blue gourami, Trichogaster trichopterus. An immersion challenge model was utilized to mimic natural infection conditions in fish. Gastrointestinal tract, gills and the body surface of fish were found to be the sites of entry of virulent E. tarda (PPD130/91) by histological and infection kinetics studies. On the other hand, avirulent E. tarda (PPD125/87) was mainly found in the gastrointestinal tract, and the bacterial population in tissue declined over a period of 7 days.

AB - The application of green fluorescent protein (GFP) to identify the portal(s) of entry of bacterial pathogens in animal hosts was studied using the fish pathogen Edwardsiella tarda and blue gourami, Trichogaster trichopterus. An immersion challenge model was utilized to mimic natural infection conditions in fish. Gastrointestinal tract, gills and the body surface of fish were found to be the sites of entry of virulent E. tarda (PPD130/91) by histological and infection kinetics studies. On the other hand, avirulent E. tarda (PPD125/87) was mainly found in the gastrointestinal tract, and the bacterial population in tissue declined over a period of 7 days.

KW - Edwardsiella tarda

KW - Green fluorescent protein

KW - Infection kinetics

KW - Portal of entry

UR - http://www.scopus.com/inward/record.url?scp=0035862446&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(00)00498-5

DO - 10.1016/S0378-1097(00)00498-5

M3 - 文章

C2 - 11164315

AN - SCOPUS:0035862446

SN - 0378-1097

VL - 194

SP - 239

EP - 243

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Green fluorescent protein-tagged Edwardsiella tarda reveals portal of entry in fish

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this