Green fluorescent protein-tagged Edwardsiella tarda reveals portal of entry in fish

Sharon H.M. Ling, Xian Hui Wang, Tit Meng Lim, Ka Yin Leung*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

The application of green fluorescent protein (GFP) to identify the portal(s) of entry of bacterial pathogens in animal hosts was studied using the fish pathogen Edwardsiella tarda and blue gourami, Trichogaster trichopterus. An immersion challenge model was utilized to mimic natural infection conditions in fish. Gastrointestinal tract, gills and the body surface of fish were found to be the sites of entry of virulent E. tarda (PPD130/91) by histological and infection kinetics studies. On the other hand, avirulent E. tarda (PPD125/87) was mainly found in the gastrointestinal tract, and the bacterial population in tissue declined over a period of 7 days.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalFEMS Microbiology Letters
Volume194
Issue number2
DOIs
StatePublished - 15 Jan 2001
Externally publishedYes

Keywords

  • Edwardsiella tarda
  • Green fluorescent protein
  • Infection kinetics
  • Portal of entry

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