Genetic insights unraveling quorum quenching potential of indigenous isolates from an anaerobic membrane bioreactor

Hira Waheed, Ch. Tahir Mehmood, Yiwei Li, Yongyu Yang, Yeyuan Xiao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Despite a few reports of quorum quenching (QQ) in anaerobic membrane bioreactors (AnMBRs), the sensing, regulation and degradation mechanism for quorum sensing (QS) signals by indigenous QQ isolates have been barely studied. This study employed isolation and screening of indigenous QQ strains from anaerobic sludge for acyl-homoserine lactones (AHLs) degradation and membrane biofouling control. High-quality whole genome sequences of Micrococcus luteus anQ-m1, Bacillus pacificus anQ-h4, and Lysinibacillus capsici anQ-h6 were obtained, with a genome size of 2.5, 5.6, and 4.7 Mbp, respectively. Amidase-encoding amiE was the only QQ gene in anQ-m1, while anQ-h6 carries both amiE and lactonase-encoding aiiB genes. Genes responsible for QS autoinducer synthesis were not identified in anQ-m1 and anQ-h6, suggesting low potential of biofilm promotion via QS. Despite a peptidic QS system responsible for biofilm formation, anQ-h4 bears the most comprehensive QQ system, including amiE-amidase, aiiA-lactonase, CYP102A5-cytochrome oxidoreductase, and lsrK-autoinducer-2 kinase. This study elucidates QS and QQ mechanisms of potential anaerobes and provides fundamentals for designing QQ consortia to effectively control biofouling in AnMBRs.
Original languageEnglish
JournalScience of the Total Environment
DOIs
StatePublished - 10 Mar 2022

Keywords

  • Biofouling control
  • Anaerobic quorum quenching
  • Whole-genome sequencing
  • Micrococcus luteus
  • Bacillus pacificus
  • Lysinibacillus capsici

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