Functional dominance and community compositions of ammonia-oxidizing archaea in extremely acidic soils of natural forests

Ruo Nan Wu, Han Meng, Yong Feng Wang, Ji Dong Gu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Extremely acidic soils of natural forests in Nanling National Nature Reserve have been previously investigated and revisited in two successive years to reveal the active ammonia oxidizers. Ammonia-oxidizing archaea (AOA) rather than ammonia-oxidizing bacteria (AOB) were found more functionally important in the extremely acidic soils of the natural forests in Nanling National Nature Reserve. The relative abundances of Nitrosotalea, Nitrososphaera sister group, and Nitrososphaera lineages recovered by ammonia monooxygenase subunit A (amoA) transcripts were reassessed and compared to AOA communities formerly detected by genomic DNA. Nitrosotalea, previously found the most abundant AOA, were the second-most-active lineage after Nitrososphaera sister group. Our field study results, therefore, propose the acidophilic AOA, Nitrosotalea, can better reside in extremely acidic soils while they may not contribute to nitrification proportionately according to their abundances or they are less functionally active. In contrast, the functional importance of Nitrososphaera sister group may be previously underestimated and the functional dominance further extends their ecological distribution as little has been reported. Nitrososphaera gargensis–like AOA, the third abundant lineage, were more active in summer. The analyses of AOA community composition and its correlation with environmental parameters support the previous observations of the potential impact of organic matter on AOA composition. Al 3+ , however, did not show a strong adverse correlation with the abundances of functional AOA unlike in the DNA-based study. The new data further emphasize the functional dominance of AOA in extremely acidic soils, and unveil the relative contributions of AOA lineages to nitrification and their community transitions under the environmental influences.

Original languageEnglish
JournalApplied Microbiology and Biotechnology
DOIs
StateAccepted/In press - 2019
Externally publishedYes

Keywords

  • Aluminum
  • Ammonia monooxygenase subunit a (amoA)
  • Ammonia-oxidizing archaea (AOA)
  • Nitrososphaera sister group
  • Nitrosotalea
  • Organic matter

Fingerprint Dive into the research topics of 'Functional dominance and community compositions of ammonia-oxidizing archaea in extremely acidic soils of natural forests'. Together they form a unique fingerprint.

Cite this