Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB

J. Paul Chen; Shunnian Wu

doi:10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P

Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB

J. Paul Chen, Shunnian Wu

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified from baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was ~90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.

Original language	English
Pages (from-to)	798-802
Number of pages	5
Journal	Journal of Chemical Technology and Biotechnology
Volume	75
Issue number	9
DOIs	https://doi.org/10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P
State	Published - Sep 2000
Externally published	Yes

Keywords

Alcohol dehydrogenase
CTAB
Protein purification
Reversed micelles

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P

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abstract = "A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified from baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was ~90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.",

keywords = "Alcohol dehydrogenase, CTAB, Protein purification, Reversed micelles",

author = "{Paul Chen}, J. and Shunnian Wu",

year = "2000",

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doi = "10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P",

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T1 - Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB

AU - Paul Chen, J.

AU - Wu, Shunnian

PY - 2000/9

Y1 - 2000/9

N2 - A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified from baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was ~90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.

AB - A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified from baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was ~90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.

KW - Alcohol dehydrogenase

KW - CTAB

KW - Protein purification

KW - Reversed micelles

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U2 - 10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P

DO - 10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P

M3 - 文章

AN - SCOPUS:0034284113

VL - 75

SP - 798

EP - 802

JO - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 9

ER -

Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB

Abstract

Keywords

UN SDGs

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