TY - JOUR
T1 - Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB
AU - Paul Chen, J.
AU - Wu, Shunnian
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2000/9
Y1 - 2000/9
N2 - A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified from baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was ~90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.
AB - A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified from baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was ~90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.
KW - Alcohol dehydrogenase
KW - CTAB
KW - Protein purification
KW - Reversed micelles
UR - http://www.scopus.com/inward/record.url?scp=0034284113&partnerID=8YFLogxK
U2 - 10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P
DO - 10.1002/1097-4660(200009)75:9<798::AID-JCTB282>3.0.CO;2-P
M3 - 文章
AN - SCOPUS:0034284113
VL - 75
SP - 798
EP - 802
JO - Journal of Chemical Technology and Biotechnology
JF - Journal of Chemical Technology and Biotechnology
SN - 0268-2575
IS - 9
ER -