Extraction of yeast alcohol dehydrogenase using reversed micelles formed with CTAB

J. Paul Chen, Shunnian Wu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A large molecular weight protein, alcohol dehydrogenase (ADH, 141 kDa), has been purified from baker's yeast using reversed micelles formed with a cationic surfactant (cetyltrimethylammonium bromide, CTAB) using the phase transfer method. Various parameters, such as pH, ionic strength and contact time of the two phases in ADH forward and backward transfer were studied. The active ADH was successfully recovered after a full forward and backward extraction cycle. The recovery of ADH activity obtained was ~90% and the purification factor was 3.1 for the overall process. (C) 2000 Society of Chemical Industry.

Original languageEnglish
Pages (from-to)798-802
Number of pages5
JournalJournal of Chemical Technology and Biotechnology
Volume75
Issue number9
DOIs
StatePublished - Sep 2000
Externally publishedYes

Keywords

  • Alcohol dehydrogenase
  • CTAB
  • Protein purification
  • Reversed micelles

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